Why are HAP1 cells a great option as a gene-edited cell line model?
The HAP1 cell line has one copy of each gene, ensuring the edited allele will not be masked by additional alleles
This cell line also allows for multiple knockouts in the same model, enabling studies of additive knockout effects
HAP1 cell line properties
- Sustains growth at single cell dilutions
- Ease of transfection
- Rapid doubling time
HAP1 is a human near-haploid cell line derived from the male chronic myelogenous leukemia (CML) cell line KBM-7.
Human HAP1 Parental Cell Line
Cat # C631
- Wild-type HAP1 cells NOT SELECTED for ploidy status.
- C631 parental HAP1 cells are the parental cell line for all of our HAP1 knockout cell lines. It is what we use to generate the knockout cell lines, and it is also what we send along with all KO cell lines as the parental control.
Human HAP1 parental cell line (screening ready)
Cat # C859
- Quality-controlled near-haploid HAP1 wild-type cells SELECTED for ploidy status.
Human KBM-7 cell line
Cat # C628
- Quality-controlled near-haploid KBM-7 wild-type cells.
- All wild-type HAP1 cells were derived from the KBM-7 cell line.
Human eHAP cell line
Cat # C669
- Quality-controlled fully-haploid engineered HAP1 (eHAP) wild-type cells
- eHAP cells are completely haploid. We have used genome engineering to remove the fragment of chromosome 15 from chromosome 19, so no genes are duplicated in this cell line. This cell line does still have the Philadelphia chromosome