siGLO RISC-Free Control

Isolate cellular effects related only to siRNA transfection

A stable, fluorescent RNAi control that does not engage RISC and permits isolation of transfection-related cellular effects during siRNA experiments in human, mouse, or rat cells.
Dharmacon

siGLO RISC-Free Control siRNA is chemically modified to impair processing and uptake by RISC, allowing researchers to isolate cellular effects related to siRNA transfection. As transfection reagents typically rely on charged interaction with the nucleic acid payload, transfection controls using siGLO RISC-Free are recommended over the use of transfection reagent alone.

Highlights
  • Stable, fluorescent, negative RNAi control
  • Labeled with Cy3
  • Absorbance/Emission Max is 547/563 nm
  • A Cy3, Rhodamine or PE filter can be used
  • Not processed by RISC - useful to isolate cellular effects related only to siRNA transfection
  • Enables co-transfection with functional siRNA without interfering with target gene silencing

To request a larger quantity or in vivo processing of this control product, please contact scientific support.