Trans-Lentiviral shRNA Packaging System
Package 2nd or 3rd generation viral vectors with high efficiency. Lentiviral packaging kits with calcium phosphate provide lower cost and higher-efficiency viral packaging along with premium biosafety.
Please Note: The HEK293T cells are being discontinued as of July 2023. Please contact your local Horizon Discovery representative for information on ordering these HEK293T cells as part of the kit for purchase on Horizon Discovery website.
The Trans-Lentiviral Packaging System utilizes the most advanced strategy for generating lentiviral particles for transduction of a gene or shRNA of interest. Co-transfection of the Trans-Lentiviral packaging plasmid mix and transfer vector into the packaging cell line, HEK293T, allows efficient production of lentiviral supernatant. Lentiviral particles can deliver genetic content into a wide range of cell types, including both dividing and non-dividing mammalian cells (see Figure 1, Supporting Data tab).
Highlights
- Consistent titers: 1 to 5 x 106 transducing units (TU) per mL are commonly achieved in collected supernatant
- Broad tropism: Effectively transduce most mammalian cell lines, including primary and non-dividing cells
- Patented technology: Contains all genetic elements necessary to package both second and third generation lentiviral vectors
Highest Level of Biosafety
- Five separate plasmids: Genes required for packaging the viral genome are separated onto five plasmids to minimize the threat of recombinant, replication-competent virus production
- Replication-incompetent: None of the structural genes are actually present in the packaged viral genome, therefore no new replication-competent virus can be produced
Product Formats
- With or without HEK293T cells
- Starter kits (10rxns) with an experimental control
- Bulk sizes (50, 100rxns) for large experiments
Trans-Lentiviral shRNA Packaging Kit Components
| Kit Contents | TLP5912 (10rxn) | TLP5917 (10rxn + cells) | TLP5913 (50rxn) | TLP5914 (100rxn) |
|---|---|---|---|---|
| Trans-Lentiviral Packaging Mix | 285µg | 285µg | 285µg x 5 | 285µg x 10 |
| HEK293T Packaging Cell Line | not included | 1.0mL | not included | not included |
| CaCl2 Reagent | 1.2mL | 1.2mL | 6mL | 6mL x 2 |
| 2X HBSS Reagent | 12.0mL | 12.0mL | 60mL | 60mL x 2 |
| pGIPZ Non-Silencing Control Vector DNA | 45 µg (0.45µg/µL) | 45µg (0.45µg/µL) | not included | not included |
Note: All plasmids are shipped on wet ice and stored at -20°C. For kits containing the packaging cell line, HEK293T, an additional dry ice cooler will be included in the shipment. Upon receipt, the cell line must be stored in liquid nitrogen for two days.
