In functional testing, the GFP Duplex I control siRNA has demonstrated high efficiency silencing of the green fluorescent protein reporter gene (GFP/eGFP) at both the mRNA and protein levels.
Because the GFP reporter sequence has been altered by various sources to improve its performance, we recommend aligning the siRNA target sequence to your reporter to ensure a sequence match. This siRNA control does not target TurboGFP.
- Target sequence: 5’-
GCA AGC TGA CCC TGA AGT TC–3’
- Appropriate for use in any mammalian cell type
- N. J. Caplen, S. Parrish, F. Imani, A. Fire, R. A. Morgan, Specific inhibition of gene expression by small double-stranded RNAs in invertebrate and vertebrate systems. Proc Natl Acad Sci U S A. 98, 9742-9747 (14 August 2001).
|Storage Conditions||-20 C|
|Stability at Recommended Storage Conditions||At least 12 months|