siRNA control recommendations
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Predesigned siRNA product lines
Guaranteed to silence target gene expression by at least 75%
Use our online design tool to create and order a custom siRNA specific for your application.
siRNA knockdown experiments are the gold standard for transiently assessing a protein’s function. Researchers employing this powerful technology need confidence in 1) the functional silencing of their gene of interest and 2) careful mitigation of off-target effects.
Scientists from Dharmacon™ (now part of the Horizon team) pioneered siRNA design rules 20 years ago and have been perfecting algorithms for high potency silencing ever since. Horizon continues to innovate the siRNA product portfolio to offer unmatched efficiency, accuracy, and deliverability.
The SMARTselection algorithm was adapted from A. Birmingham et. al., A protocol for designing siRNAs with high functionality and specificity, Nature Methods., Aug 2007; 9: 2068-2078.
All siRNA reagents are available individually, as sets of 4 individual siRNAs, or as a SMARTpool.
The SMARTpool combines four gene-specific siRNAs into a single reagent. Research has shown that pooled siRNAs:
siRNA knockdown experiments only advance your research when there is a high degree of confidence that the desired gene is the only gene silenced. The primary source of siRNA-mediated off-targets is the seed region (nucleotides 2-7) which uses the microRNA pathway to induce non-specific gene silencing via interactions within the 3' UTR. Horizon’s SMARTselection algorithm incorporates comprehensive bioinformatic strategies like seed region filters and seed frequency analysis within its design strategy to reduce microRNA-like off-target activity.
Choosing a Dharmacon siRNA product line and format