The Andrews lab at the University of Toronto has developed a collection of more than 5,000 yeast strains that each overexpresses a different yeast open reading frame (ORF) when induced with galactose.
More than 80% of the S. cerevisiae genome is represented. ORFs are plasmid-encoded, tagged at the N-terminus with GST, and expressed under control of the GAL1/10 promoter (Zhu et al, 2001). The Yeast GST-tagged ORFs is in a BY4741 (MATa) background and has been used for genome-scale overexpression screening (Sopko et al, 2006).
- More than 5,000 yeast strains overexpress an ORF through galactose induction
- Collection represents more than 80% of the S. cerevisiae genome
We provide certain clone resources developed by leading academic laboratories. Many of these resources address the needs of specialized research communities not served by other commercial entities. In order to provide these as a public resource, we depend on the contributing academic laboratories for quality control.
Therefore, these are distributed in the format provided by the contributing institution "as is" with no additional product validation or guarantee. We are not responsible for any errors or performance issues. Additional information can be found in the product manual as well as in associated published articles (if available). Alternatively, the source academic institution can be contacted directly for troubleshooting.
Yeast strains are shipped in CM broth with glucose–uracil (Teknova cat # C7141) with 15% glycerol (added after growth of strains). Individual strains are shipped in 2 mL tubes at room temperature; the entire collection is shipped in 96-well plates on dry ice. All strains should be stored at –80°C.
- R. Sopko et al., Mapping pathways and phenotypes by systematic gene overexpression. Molecular Cell. 21(3), 319–330 (3 February 2006).
- H. Zhu et al., Global analysis of protein activities using proteome chips. Science. 293(5537), 2101–2105 (14 September 2001).