Self-delivering siRNA for difficult-to-transfect cells
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Accell siRNA achieves what no other product can claim: delivery into difficult-to-transfect cells without additional reagents, virus, or instruments. Achieve guaranteed gene silencing in cells that had been beyond the reach of conventional RNAi methods due to toxicity from transfection reagents, electroporation, or undesirable viral responses.
- Novel, patented siRNA modifications facilitate passive uptake, protection against nuclease degradation, specificity, and knockdown efficiency
- Accell self-delivering siRNA enters cells without the need for transfection reagents, viral vector transduction, or instruments (nucleofection or electroporation)
- Low toxicity enables extended-duration silencing from multiple doses – up to 30 days!
Proven performance in difficult-to-transfect cell types
- Immunological cells – Jurkat cells, primary T cells, lymphocytes, mononuclear cells (PBMC), macrophages
- Neuronal cells – primary neurons, glioma cells, organotypic brain slices
- Primary cells – fibroblasts, cardiomyocytes, β-islet cells
- In vivo models – brain delivery, dermal (skin) delivery, periodontal model
The Accell siRNA application protocol simplifies targeted gene knockdown
(1) Combine Accell siRNA with Accell delivery media (or other low- or no-serum media) (2) Add Accell delivery mix directly to cells and incubate for 72 hours.
This breakthrough in siRNA delivery requires no transfection reagent, but has some unique application requirements.
- Accell siRNA works at a higher concentration than conventional siRNA; recommended 1µM working concentration.
- Delivery may be inhibited by the presence of BSA in serum. Optimization studies with serum-free media formulations (Accell Delivery Media) or < 2.5% serum in standard media is recommended.
- Full-serum media can be added back after 48 hours of incubation, optimal mRNA silencing is typically achieved by 72 hours, or up to 96 hours for protein knockdown.
Our siRNA knockdown guarantee
Accell siRNA reagents (SMARTpool and three of four individual siRNAs) are guaranteed to silence target gene expression by at least 75% at the mRNA level when used under optimized conditions as defined in this featured article.
Accell siRNA format options
A mixture of 4 siRNA provided as a single reagent; providing advantages in both potency and specificity.
Set of 4:
A convenient option for purchasing aliquots of all 4 individual siRNAs targeting a single gene.
Select 1, 2, 3 or 4 individual siRNAs per gene.
|Approximate # reactions (wells) at 1 µM siRNA concentration (assuming no loss from pipetting)|
(100 uL total reaction volume)
(500 uL total reaction volume)
(1000 uL total reaction volume)
Due to the unique nature of Accell siRNA delivery, it requires a higher working concentration than conventional siRNAs. This table provides the approximate number of reactions (wells) at recommended 1μM Accell siRNA working concentration in different plate formats (assuming no loss from pipetting).
Effectively optimize Accell siRNA delivery conditions and control for variables in ongoing experiments with fluorescent or unlabeled positive and negative controls. Accell Control Kits combine species-specific validated controls for assessment of Accell technology in your difficult-to-transfect cells. Choose from our selection of positive controls (targeting housekeeping genes), fluorescent or unlabeled negative controls, or an Accell Control Kit. Although Accell siRNA requires no transfection reagent for delivery, it is recommended for use at 1 µM concentration, so be sure to adjust order quantity accordingly.
Accell positive control reagents
Validated positive control siRNA targeting the Cyclophilin B (PPIB) housekeeping gene in human, mouse, or rat. Accell siRNA requires no transfection reagent or viral vector for delivery into difficult-to-transfect cells.
Validated positive control pool of four siRNAs targeting the Cyclophilin B (PPIB) housekeeping gene in human, mouse, or rat. Accell siRNA requires no transfection reagent or viral vector for delivery into difficult-to-transfect cells.
Validated positive control siRNA targeting the GAPD housekeeping gene in human, mouse, or rat. Accell siRNA requires no transfection reagent or viral vector for delivery into difficult-to-transfect cells.
Validated positive control pool of four siRNAs targeting the GAPD housekeeping gene in human, mouse, or rat. Accell siRNA requires no transfection reagent or viral vector for delivery into difficult-to-transfect cells.
Validated, fluorescent positive control siRNA targeting the PPIB housekeeping gene in human, mouse, or rat. Cytoplasmic presence of label (FAM) permits qualitative assessment of Accell siRNA passive delivery.
Validated, fluorescent positive control siRNA targeting the PPIB housekeeping gene in human, mouse, or rat. Cytoplasmic fluorescence permits qualitative assessment of Accell siRNA passive delivery.
Validated, fluorescent positive control siRNA targeting the eGFP housekeeping gene in human, mouse, or rat. Cytoplasmic fluorescence permits qualitative assessment of Accell siRNA passive delivery.
Validated positive control siRNA pool targeting the green fluorescent protein (eGFP) gene for use in cells from any species. Accell siRNA requires no transfection reagent or viral vector for delivery into difficult-to-transfect cells.
Accell negative control reagents
A negative control siRNA to determine baseline cellular response to Accell siRNA application. Accell siRNA requires no transfection reagent or viral vector for delivery into difficult-to-transfect cells.
A negative control pool of four siRNAs to determine baseline cellular response to Accell siRNA application. Component siRNAs are designed to target no human, mouse, or rat genes.
Fluorescent negative control siRNA for assessment of Accell passive delivery technology as determined by cytoplasmic localization of the FAM-labeled Accell siRNA. Designed to target no human, mouse, or rat genes.
Fluorescent negative control siRNA for reliable assessment of Accell passive delivery technology as determined by cytoplasmic localization of the DY-547-labeled Accell siRNA. Designed to target no human, mouse, or rat genes.
Accell control siRNA kits
A kit containing four validated species-specific positive, negative, and FAM-labeled siRNA controls to assess Accell self-delivering siRNA technology in your difficult-to-transfect cells. Includes resuspension buffer and Accell delivery media.
A kit containing four species-specific validated positive, negative,and DY-547-labeled siRNA controls to assess Accell self-delivering siRNA technology in your difficult-to-transfect cells. Includes resuspension buffer and Accell delivery media.
Accell siRNA libraries
Find the predefined gene family, including Druggable genes or Whole Genome, that’s right for your discovery efforts.
A wide selection of predefined siRNA libraries, including the most up-to-date Whole Mouse genome collection available.
Fast and easy online configuration and ordering of plated siRNA & microRNA reagents targeting your genes of interest.
No pre-designed product to fit your needs? Use our online design tools and extensive synthesis options to create a custom siRNA specific for your application.
Researchers have submitted the following figures to show off their great gene silencing results in their difficult-to-transfect cells using Accell siRNA. If you have data to showcase, fill out this brief form and you will receive instructions on how to submit your data! New data will be added regularly, so check back often!
RNA interference by the Accell system in human skin mast cells
Mast cells were purified from human skin of healthy donors according to our routine protocol (e.g. PMID: 14634065, 15191551, 15666093, 15675967, 20545757, 24671954, 25725371, 26706922, 28264498, 28845295, 28859248), reaching 98-100% purity, and transfected with siRNA as specified by the Dharmacon; all siRNAs were used at 1 µM. (A) Cellular uptake of siRNA, as measured by fluorescence microscopy using PE-labeled (non-targeting) siRNA (24 h after transfection). (B) and (C) relative gene expression in cells transfected with non-targeting siRNA versus siRNA targeting either GAPDH or Cyclophilin B, measured by RT-qPCR (48 h post-transfection) and normalized to control expression given as 1. Mean ± SEM of n = 6-9 individual experiments. Note that the knockdown was both highly efficient and specific, as only the targeted RNA was downregulated, while the Cyclophilin B-specific siRNA had no impact on GAPDH expression and vice versa. ** p < 0.01; *** p < 0.01; ****p < 0.0001 by Kruskal-Wallis test with multiple comparisons (GraphPad Prism 7.0 software). NS = not significant.
Submitted by Magda Babina, PhD
Department of Dermatology and Allergy, Universitätsmedizin Berlin
Accell siRNA delivery and gene silencing in cardiomyocytes
Neonatal rat ventricular myocytes were incubated with 1 μM Accell Green (A; Cat# D-001950-01) or Red (B; Cat# D-001960-01) Non-targeting siRNA for 72 hours in Accell delivery media (Cat# B-005000). Nuclei were stained with DAPI (blue). Labeled control uptake showed diffuse cytoplasmic localization in nearly all cells. The bar graph indicates the level of gene silencing achieved with Accell GAPD Control siRNA (Cat# D-001930-03) and Pool (Cat# D-001930-30) control reagents when used with neonatal rat ventricular myocyte (NRVM) media or Accell delivery media. Myocytes were prepared as described in Maass AH & Buvoli M. Cardiomyocyte preparation, culture, and gene transfer. Methods in Molecular Biology 2007;366: 321-30. mRNA expression was determined by QuantiGene branched DNA assay (Panomics).
Accell siRNA reagents are specially modified for use in difficult-to-transfect cells without the need for transfection reagents, virus, or electroporation. The following selected peer-reviewed publications have cited their successful use in a variety of experimental systems.
For In vivo publications, please see our In vivo siRNA reading list.
Established cell lines
ARPE-19 (human retinal epithelial cells) - 39
BxPC3 (pancreatic tumor cell lines) - 9
C1 tumor derived cells - 52
Caco-2 (colon colorectal adenocarcinoma) - 27
DG-75 human B lymphocytes – 78
GH3 (rat somatolactotrophs pituitary cell line) - 62
H9 stem cell lines - 49
HCT-116 (colorectal carcinoma) - 28
HUVEC - 29
JJN3(plasma cell leukemia) - 44
KG1 (human acute myelogenous leukemia (AML) macrophage cell line) - 68
LUHMES (Lund human mesencephalic cells) - 69
MEC1 (human chronic B cell leukemia) - 14
MN-1 - 35
MS1 (mouse pancreatic islet endothelia cells) – 22
NOD CD4+CD25− splenic cells - 41
NOXA - 44
OVCA 420 (ovarian carcinoma) - 58
PGA-1 (lymphocytic leukemia B cell line) - 67
RAW264.7 macrophages - 55
SKBR3 (ER-/PR-/HER2+ breast cancer cell line) - 72
SNB19 glioma cells - 13
T47D (ductal breast epithelial tumor cell line) - 23
T98 glioma cells - 13
U266 (peripheral blood B lymphocyte myeloma) - 44
U937 (leukemic monocyte lymphoma) - 54
Primary cells & in vivo
β-islet cells - 15
Cardiomyocytes - 5
Colon stem/progenitor cells - 76
Corneal endothelial cells (adult human CECs), and ex vivo human corneal endothelium - 75
Endometrial cells - 16
Extravillous trophoblasts (EVT) - 32
Fibroblasts (primary) - 73
Immortalized B cells - 65
Keratinocytes - 57
Lung epithelial cells - 80
Lymphocytes - 47
Mantle cell lymphoma cells (MCL) - 48
Monocytes - 19
Mouse embryonic fibroblasts (MEF) - 24
Naïve fetal T cells - 82
Natural killer (NK) cell line - 61
Neonatal mouse ovary - 81
Neurons (primary rat) - 20
Neurons derived from iPS cells - 74
Oligodendrocyte precursors - 60
Pancreatic tumor cell lines - 9
Regulatory T cells - 83
Stem cell–derived peripheral neurons - 79
In vivo skin delivery - 18
In vivo rat periodontal model - 33
In vivo mouse intradermal injection - 43
In vivo mouse brain - 69
1. H. Mortiboys, J. Aasly, et al. Ursocholanic acid rescues mitochondrial function in common forms of familial Parkinson's disease. Brain 136, 3038-3050 (2013). [Mouse primary cortical neurons]
2. D. Gomez, K. Kessler, et al. Modifications of Chromatin Dynamics Control Smad2 Pathway Activation in Aneurysmal Smooth Muscle Cells. Circ. Res. 113, 881 – 890 (2013). [Human vascular smooth muscle cells (VSMC) ]
3. S. Jiang, D. Part, et al. Mitochondria and AMP-activated Protein Kinase-dependent Mechanism of Efferocytosis. J. Biol. Chem. 288, 26013 - 26026 (2013). [mouse primary perionatal macrophages]
4. S. Sumitomo, K. Fujio, et al. Transcription Factor Early Growth Response 3 Is Associated with the TGF-β1 Expression and the Regulatory Activity of CD4-Positive T Cells In vivo. J. Immunol. 191, 2351 - 2359 (2013). [CD4+ primary human T cells]
5. F. Beig, J. Schmeckpeper, et al. C3orf58, a Novel Paracrine Protein, Stimulates Cardiomyocyte Cell-Cycle Progression Through the PI3K–AKT–CDK7 Pathway. Circ. Research 113, 372 – 380 (2013). [neonatal cardiomyocytes]
6. T. J. Mulrooney, P. E. Posch, et al. DAP12 impacts trafficking and surface stability of killer immunoglobulin-like receptors on natural killer cells. J. Leukoc. Biology 94, 301 – 313 (2013). [PMBCs]
7. R. Arita, S. Nakao, et al. A Key Role for ROCK in TNF-α–Mediated Diabetic Microvascular Damage. Invest. Ophthalmol. Vis. Science 54, 2373 – 2383 (2013). [cultured microvascular endothelial cells]
8. S. Hannila, M. Siddiq, et al. Secretory Leukocyte Protease Inhibitor Reverses Inhibition by CNS Myelin, Promotes Regeneration in the Optic Nerve, and Suppresses Expression of the Transforming Growth Factor-β Signaling Protein Smad2. J. Neuroscience 33, 5138 – 5151 (2013). [P6 CGNs and P1 rat cortical neurons]
9. K. E. Johnson , S. Mitra , et al. Phosphorylation on Ser-279 and Ser-282 of connexin43 regulates endocytosis and gap junction assembly in pancreatic cancer cells. Mol. Biol. Cell 24, 715 – 733 (2013). [human pancreatic tumor cell lines BxPC3]
10. S. Yasunaga, M. Ohtsubo et al. Scmh1 Has E3 Ubiquitin Ligase Activity for Geminin and Histone H2A and Regulates Geminin Stability Directly or Indirectly via Transcriptional Repression of Hoxa9 and Hoxb4. Mol. Cell. Biology 33, 644 – 660 (2013). [mouse BM cells]
11. G. Lopez-Castejon, N. M. Luheshi, et al. Deubiquitinases Regulate the Activity of Caspase-1 and Interleukin-1β Secretion via Assembly of the Inflammasome. J. Biol. Chem 288, 2721 – 2733 (2103). [THP-1]
12. G. R. Tundo, D. Sbardella, et al. Insulin-degrading Enzyme (IDE): A NOVEL HEAT SHOCK-LIKE PROTEIN. J. Biol. Chemistry 288, 2281 – 2289 (2013). [SHSY5Y cells]
13. H. J. Seol, J. H. Chang, et al. Overexpression of CD99 Increases the Migration and Invasiveness of Human Malignant Glioma Cells. Genes & Cancer 3, 535 – 549 (2012). [T98 and SNB19 glioma cells]
14. A. G. Ramsay, A. J. Clear, et al. Multiple inhibitory ligands induce impaired T-cell immunologic synapse function in chronic lymphocytic leukemia that can be blocked with lenalidomide: establishing a reversible immune evasion mechanism in human cancer. Blood 120, 1412 – 1421 (2012). [MEC1 cells – Human chronic B cell leukemia]
15. M.R. Metukuri, P. Zhang, et al. ChREBP Mediates Glucose-Stimulated Pancreatic β-Cell Proliferation. Diabetes 61, 2004 – 2015 (2012). [human and rat islet cells]
16. E. M. De La Garza, P. A. Binkley,et al. Raf-1, a Potential Therapeutic Target, Mediates Early Steps in Endometriosis Lesion Development by Endometrial Epithelial and Stromal Cells. Endocrinology 153, 3911 – 3921 (2012). [primary endometrial cells]
17. X. Qu, G. Zhuang, et al. Induction of Bv8 Expression by Granulocyte Colony-stimulating Factor in CD11b+Gr1+ Cells. J. Biol. Chemistry 287, 19574-19584 (2012). [primary mouse bone marrow cells]
18. R. P. Hickerson, M. A. Flores, et al. Use of self-delivery siRNAs to inhibit gene expression in an organotypic pachyonychia congenita model. J Invest Dermatol 131(5), 1037-44 (2011) [In vivo skin delivery]
19. A. Nijnik, J. Pistolic, et al. The role of the Src family kinase Lyn in the immunomodulatory activities of cathelicidin peptide LL-37 on monocytic cells. J. Leukoc. Biology 91, 599-607 (2012) [monocytes]
20. A. Vagnoni, M. S. Perkinton, et al. Calsyntenin-1 mediates axonal transport of the amyloid precursor protein and regulates Aβ production. Human Molecular Genetics 21(13), 2845-54 (2012). [Rat E18 primary neurons]
21. P. Kuo, M. Huang, et al. Lung Cancer-derived Galectin-1 Enhances Tumorigenic Potentiation of Tumor-associated Dendritic Cells by Expressing Heparin-binding EGF-like Growth Factor. J. Biol. Chem. 287, 9753-9764 (2012). [CD14+ primary monocytes]
22. M. Franco, P. Roswall, et al. Pericytes promote endothelial cell survival through induction of autocrine VEGF-A signaling and Bcl-w expression. Blood 118, 2906 – 2917 (2011). [MS1cells; Mouse pancreatic islet endothelia]
23. A. A. Fiorillo, T. R. Medler, et al. HMGN2 Inducibly Binds a Novel Transactivation Domain in Nuclear PRLr to Coordinate Stat5a-Mediated Transcription. Molecular Endocrinology 25, 1550 – 1564 (2011). [T47D cells]
24. C. X. George and C. E. Samuel, Host Response to Polyomavirus Infection Is Modulated by RNA Adenosine Deaminase ADAR1 but Not by ADAR2. Journal of Virology 85, 8338 – 8347 (2011). [MEF cells]
25. M. Meguro-Horike, D. H. Yasui, et al. Neuron-specific impairment of inter-chromosomal pairing and transcription in a novel model of human 15q-duplication syndrome. Human Molecular Genetics 20(19), 3798-810 (2011). [SH-SY5Y]
26. Emily Turner-Brannen, Ka-Yee Grace Choi, et al. Inflammatory Cytokines IL-32 and IL-17 Have Common Signaling Intermediates despite Differential Dependence on TNF-Receptor 1. J. Immunology 186, 7127 – 7135 (2011). [THP-1 monocytes]
27. R. Al-Sadi, K. Khatib, et al. Occludin regulates macromolecule flux across the intestinal epithelial tight junction barrier. Am J Physiol Gastrointest Liver Physiology 300, G1054 - G1064 (2011). [Caco-2 cells]
28. R. J. Boohaker, G. Zhang, et al. BAX supports the mitochondrial network, promoting bioenergetics in nonapoptotic cells. Am J Physiol Cell Physiology 300, C1466-C1478 (2011). [HCT-116]
29. K. He, G. Sui, et al. Feedback Regulation of Endothelial Cell Surface Plasmin Generation by PKC-dependent Phosphorylation of Annexin A2. J. Biol. Chemistry 286, 15428-15439 (2011). [HUVEC cells]
30. P. L. Kuo, M. S. Huang, et al. Signaling pathway of isophorone diisocyanate-responsive interleukin-8 in airway smooth muscle cells. Eur. Respir. J 37, 1226 – 1236 (2011). [BSMCs]
31. P. Kuo, Y. Hsu, et al. Bronchial Epithelium–Derived IL-8 and RANTES Increased Bronchial Smooth Muscle Cell Migration and Proliferation by Krüppel-like Factor 5 in Areca Nut–Mediated Airway Remodeling. Toxicol. Sci. 121, 177-190 (2011). [BSMCs]
32. K. Biadasiewicz, S. Sonderegger, et al. Transcription Factor AP-2α Promotes EGF-Dependent Invasion of Human Trophoblast. Endocrinology 152, 1458 – 1469 (2011). [primary EVTs]
33. Q.Li, H. Yu,et al. Silencing Mitogen-Activated Protein Kinase-Activated Protein Kinase-2 Arrests Inflammatory Bone Loss. J. Pharmacol. Exp. Ther 336, 633 – 642 (2011). [ex vivo and In vivo rat periodontal model]
34. K. Billot, J. Soeur, et al. Deregulation of Aiolos expression in chronic lymphocytic leukemia is associated with epigenetic modifications. Blood 117, 1917 – 1927 (2011). [PBMCs]
35. L. Hubers, H. Valderrama-Carvajal, et al. HuD interacts with survival motor neuron protein and can rescue spinal muscular atrophy-like neuronal defects. Hum. Mol. Genetics 20, 553 – 579 (2011). [MN-1 cells]
36. P. Kuo, J. Hung,Lung, et al. Cancer-Derived Galectin-1 Mediates Dendritic Cell Anergy through Inhibitor of DNA Binding 3/IL-10 Signaling Pathway. Journal of Immunology 186, 1521 – 1530 (2011). [Monocytes]
37. S. F. Leicht, T. M. Schwarz, et al. Adiponectin Pretreatment Counteracts the Detrimental Effect of a Diabetic Environment on Endothelial Progenitors. Diabetes 60, 652 – 661 (2011). [endothelial colony-forming cells (ECFC)]
38. D. N. Petrusca, Y. Gu, et al. Sphingolipid-mediated Inhibition of Apoptotic Cell Clearance by Alveolar Macrophages. Journal of Biological Chemistry 285, 40322 – 40332 (2010). [Rat alveolar macrophages (AM)]
39. A. Giddabasappa, M. Bauler, et al. 17-β Estradiol Protects ARPE-19 Cells from Oxidative Stress through Estrogen Receptor-β. Invest. Ophthalmol. Vis. Sci. 51, 5278 – 5287 (2010). [Human retinal epithelial cells ARPE-19 cells]
40. E.Murakami et. al. Mechanism of Activation of PSI-7851 and its Diastereoisomer PSI-7977. Journal of Biological Chemistry 285, 34337-34347 (2010). [primary human hepatocytes]
41. J. Zhang et al. MEKK3 Overexpression Contributes to the Hyperresponsiveness of IL-12–Overproducing Cells and CD4+ T Conventional Cells in Nonobese Diabetic Mice. Journal of Immunology 185, 3554 – 3563 (2010). [NOD CD4+CD25− splenic cells]
42. M. Liao et al. Inhibition of Hepatic Organic Anion-transporting Polypeptide by RNA Interference in Sandwich-cultured Human Hepatocytes: An in vitro Model to Assess Transporter-mediated Drug-drug Interactions. Drug Metabolism and Deposition 38,9 1612-1622 (2010). [freshly isolated human hepatocytes]
43. E. Gonzalez-Gonzalez et al. Silencing of Reporter Gene Expression in Skin Using siRNAs and Expression of Plasmid DNA Delivered by a Soluble Protrusion Array Device (PAD). Molecular Therapy 18(9), 1667-74 (2010). [mouse intradermal injection]
44. B. Tunquist et al. Mcl-1 Stability Determines Mitotic Cell Fate of Human Multiple Myeloma Tumor Cells Treated with the Kinesin Spindle Protein Inhibitor ARRY-520. Molecular Cancer Therapeutics 9, 2046 – 2056 (2010). [multiple myeloma cell lines: JJN3 NOXA in RPMI 8226 and U266]
45. S. Suzuki et al. Differential Roles of Epac in Regulating Cell Death in Neuronal and Myocardial Cells. Journal of Biological Chemistry 285, 24248 – 24259 (2010). [primary mouse cortical neurons (E15-17)]
46. S. Winning et al. Acute Hypoxia Induces HIF-Independent Monocyte Adhesion to Endothelial Cells through Increased Intercellular Adhesion Molecule-1 Expression: The Role of Hypoxic Inhibition of Prolyl Hydroxylase Activity for the Induction of NF-B. Journal of Immunology 185, 1786 -1793 (2010). [THP-1 monocytes]
47. M. Chetane et al. Interleukin-7 mediates glucose utilization in lymphocytes through transcriptional regulation of the hexokinase II gene. Am J Physiol Cell Physiol 298, C1560 - C1571 (2010). [lymphocytes]
48. S. Desai et al. PRDM1 Is Required for Mantle Cell Lymphoma Response to Bortezomib. Molecular Cancer Research 8, 907 - 918 (2010). [mantle cell lymphoma (MCL) cells]
49. S. Byas et al. Human Embryonic Stem Cells Maintain Pluripotency after E-Cadherin Expression Knockdown. FASEB J 24, lb172 (2010). [H9 stem cell lines]
50. B. Mukhopadhyay et al. Transcriptional Regulation of Cannabinoid Receptor-1 Expression in the Liver by Retinoic Acid Acting via Retinoic Acid Receptor. Journal of Biological Chemistry 285, 19002-19011 (2010). [cultured mouse hepatocytes]
51. V. Saini et al. CXC Chemokine Receptor 4 Is a Cell Surface Receptor for Extracellular Ubiquitin. Journal of Biological Chemistry 285, 15566 – 15576 (2010). [THP-1 monocytes]
52. I. Barbieri et al. Constitutively Active Stat3 Enhances Neu-Mediated Migration and Metastasis in Mammary Tumors via Upregulation of Cten. Cancer Research 70, 2558 – 2567 (2010). [C1 tumor derived cells]
53. J. W. Perry et al. Endocytosis of Murine Norovirus 1 into Murine Macrophages Is Dependent on Dynamin II and Cholesterol. Journal of Virology 84, 6163-6176 (2010). [murine macrophages]
54. Z-H. Xue et al., Integrin αMβ2 Clustering Triggers Phosphorylation and Activation of Protein Kinase Cδ that Regulates Transcription Factor Foxp1 Expression in Monocytes. Journal of Immunology 184, 3697-3709 (2010). [U937 cells; human leukemic monocyte lymphoma]
55. M. Steenport et al., Matrix Metalloproteinase (MMP)-1 and MMP-3 Induce Macrophage MMP-9: Evidence for the Role of TNF-a and Coclooxygenase-2. Journal of Immunology 183(12), 8119-27 (2009). [RAW264.7 macrophages]
56. A. DiFeo et al. KLF6-SV1 Is a Novel Antiapoptotic Protein That Targets the BH3-Only Protein NOXA for Degradation and Whose Inhibition Extends Survival in an Ovarian Cancer Model. Cancer Research. 69, 4733–41 (2009). [In vivomouse model]
57. A. Kovalenko et al. Caspase-8 Deficiency in Epidermal Keratinocytes Triggers an Imflammatory Skin Disease. Journal of Experimental Medicine 206: 2161 – 2177 (2009). [Keratinocytes]
58. C. Bartholomeusz et al. PEA-15 Induces Autophagy in Human Ovarian Cancer Cells and is Associated with Prolonged Overall Survival. Cancer Research 68, 9302-9310 (2008). [OVCA 420; ovarian carcinoma]
59. A.M. Dolga et al. TNF-alpha-mediates neuroprotection against glutamate-induced excitotoxicity via NF-kappaB-dependent up-regulation of K2.2 channels. Journal of Neurochemistry 107, 1158-1167 (2008). [mouse primary cortical neurons]
60. F. Mir and G.C. Le Breton. A Novel Nuclear Signaling Pathway for Thromboxane A2 Receptors in Oligodendrocytes: Evidence for Signaling Compartmentalization during Differentiation. Mol. Cell. Biology 28, 6329-6341 (2008). [oligodendrocyte precursors]
61. C.B Lai, Y.Zhang, et al. Creation of the two isoforms of rodent NKG2D was driven by a B1 retrotransposon insertion. Nucleic Acids Research 37(9), 3032-43 (2009). [mouse NK cell line]
62. G. A. Peters et al. The double-strand RNA-binding protein, PACT, is required for postnatal anterior pituitary proliferation. PNAS 106(26), 10696-10701 (2009). [GH3; rat somatolactotrophs (pituitary cell line) and LβT2 gonadotrophs]
63. N. Mookherjee et al. Intracellular Receptor for Human Host Defense Peptide LL-37 in Monocytes. J. Immunol. 2009; 183. 2688-2696 [THP-1; human monocytes]
64. B. Zheng et. al. Krüppel-like Factor 4 Inhibits Proliferation by Platelet-derived Growth Factor Receptor β-mediated, Not by Retinoic Acid Receptor α-mediated, Phosphatidylinositol 3-Kinase and ERK Signaling in Vascular Smooth Muscle Cells. Journal of Biological Chemistry 284 (34), 22773-22785 (2009). [VUMC; primary rat vascular smooth muscle cells]
65. D. Smirnov et. al. Genetic Analysis of Radiation-induced Changes in Human Gene Expression. Nature 459, 587-591 (2009). [immortalized B cells]
66. U. Dreses-Werringloer et. al. A Polymorphism in CALHM1 Influences Ca2+ Homeostasis, Ab Levels, and Alzheimer's Disease Risk. Cell 133, 1149–1161 (2008). [SHSY-5Y; human neuroblastoma]
67. A.M. McElligott et. al. The Novel Tubulin-Targeting Agent Pyrrolo-1,5-Benzoxazepine-15 Induces Apoptosis in Poor Prognostic Subgroups of Chronic Lymphocytic Leukemia. Cancer 69(21), 8366-75 (2009). [PGA-1; EBV-transformed chronic lymphocyctic leukemia (CLL) B cell line]
68. Z. Guo et. al. PIM inhibitors target CD25-positive AML cells through concomitant suppression of STAT5 activation and degradation of MYC oncogene. Blood 124, 1777-1789 (2014) [KG1; human AML macrophage cell line]
69. H. Xu et. al. Tau Silencing by siRNA in the P301S Mouse Model of Tauopathy. Current Gene Therapy 14, 343-351 (2014) [mouse E15 cortical neurons, LUHMES cells, In vivo mouse brain injection]
70. A. Collado-Alsina et. al. The Regulation of Synaptic Vesicle Recycling by cGMP-Dependent Protein Kinase Type II in Cerebellar Granule Cells under Strong and Sustained Stimulation J. Neuroscience. 2014; 34:8788-8799. [cerebellar granule cells]
71. M. Freeley et. al. RNAi Screening with Self-Delivering Synthetic siRNAs for Identification of Genes That Regulate Primary Human. T Cell Migration J. Biomolecular Screening. 2015; DOI: 10.1177/1087057115588288 [Primary human T-cells]
72. S. Das , G. Sondarva, et. al. Human Epidermal Growth Factor Receptor 2 (HER2) Impedes MLK3 Kinase Activity to Support Breast Cancer Cell Survival. J. Biol. Chemistry 290, 35, 21705–21712 (2015). [SKBR3 (ER-/PR-/HER2+ breast cancer cell line)]
73. J. Bruban, G. Voloudakis, et. al. Presenilin 1 is necessary for neuronal, but not glial, EGFR expression and neuroprotection via gamma-secretase-independent transcriptional mechanisms. FASEB J. 29, 3702-3712 (2015) [murine primary cortical neurons (E15.5) and primary fibroblasts]
74. M. Komatsu, H. E. Wheeler, et. al. Pharmacoethnicity in Paclitaxel-Induced Sensory Peripheral Neuropathy, Clin. Cancer Res. 21, 4337 – 4346 (2015) [Neurons derived from iPS cells]
75. J. G. Lee and M. Heur. WNT10B Enhances Proliferation through β-Catenin and RAC1 GTPase in Human Corneal Endothelial Cells. J. Biol. Chem. 290, 26752-26764 (2015) [Adult human corneal endothelial cells (CECs) and ex vivo human corneal endothelium]
76. I. Nasri, D. Bonnet, et. al. PAR2-dependent activation of GSK3β regulates the survival of colon stem/progenitor cells. Am J Physiol Gastrointest Liver Physiol 311, G221 - G236 (2016) [colon stem/progenitor cells]
77. H. Lee, J.W. Lee, et. al. Inflammation and Inflammatory Mediators in Kidney Disease: Cln 3-requiring 9 is a negative regulator of Th17 pathway-driven inflammation in anti-glomerular basement membrane glomerulonephritis Am J Physiol Renal Physiol. 311, F505-F519 (2016)
78. T. Tagawa, M. Albanese, et. al. Epstein-Barr viral miRNAs inhibit antiviral CD4+ T cell responses targeting IL-12 and peptide processing. J Exp Med. 213(10), 2065-2080 (2016) [human B cells, DG-75]
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