Attribute Overview

Active

Best CI

Best CI Error

Best CI Level

Chemical ID

Chemical Name

Coalism Average

Coalism Average Error

Coalism Excess

Coalism Log Chi Squared

Coalism Model

Coalism Parameters

Coalism Volume

Coalism Volume Error

Combination Name

Datasource

Dose Matrix

Dose Matrix Coverage

Dose Matrix Data

Dose Matrix Error

Dose Matrix Error Volume

Dose Matrix Interpolated

Dose Response Analytical Output

  • Dose Response analytical output is created for each dose response item. A dose response item exists for each individual test substance and fixed ratio mixture present as a stand-alone item or as a constituent of a dose matrix item.
  • A dose response item is comprised of a series of dose response points arranged by increasing dose. Additional output is created for individual substance characteristics provided by the datasource associated with the dose response item. Additional output is not created for any dose response item that represents a fixed ratio mixture.
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Dose Response Chart

  • The Dose Response Chart value is a figure which represents the dose response item as a chart with two series. The first series is comprised of the individual dose response points, including response value and response error estimate, arranged by increasing dose. The second series is a continuous representation of the response of the sigmoid instance selected as the best fit for the dose response item.
  • Dose Response Chart
  • Variable type (units): Figure
  • Number of values: 1
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Dose Response Classification

Dose Response Data

  • The Dose Response Data value is a block of preformatted tab-delimited text comprising the individual dose response points, including response value and response error estimate, arranged by increasing dose. For more information see Dose Response Data in section Output Data Formats.
  • Variable type (units): Text
  • Number of values: 1
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EC50

  • The EC50 value is the dose that corresponds to 50% of the max parameter of the sigmoid instance selected as the best fit for the dose response item. The EC50 value is an estimate of the dose at which the substance(s) achieve one-half of the maximum achievable response.
  • EC50
  • Variable type (units): Numeric (uM)
  • Number of values: 1
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EC90

  • The EC90 value is the dose that corresponds to 90% of the max parameter of the sigmoid instance selected as the best fit for the dose response item. The EC50 value is an estimate of the dose at which the substance(s) achieve one-half of the maximum achievable response.
  • EC90
  • Variable type (units): Numeric (uM)
  • Number of values: 1
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Error Etimate - Fit

  • The fit error estimate is the derived standard error in the Max Response – Fit, found from the range in that parameter that corresponds to an insignificant change in chi-squared goodness-of-fit.
  • Error Estimate - Fit
  • Variable type (units): Numeric
  • Number of values: 1
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GI100

GI125

GI150

GI50

GI75

Greco Average

Greco Average Error

Greco Excess

Greco Log Chi Squared

Greco Model

Greco Parameters

Greco Volume

Greco Volume Error

Hit Score

IC50

  • The IC50 value is the dose from the sigmoid instance selected as the best fit for the dose response item that corresponds to response value of 0.5 for the Measure associated with the dose response item. For more information on Measure response values see Measures in section Analytical Methods. This value is undefined for dose response items not achieving a Max Response – a Requires a Fit value of at least 0.5.
  • IC50
  • Variable type (units): Numeric
  • Number of values: 0 or 1
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IC80

  • Variable type (units): Numeric
  • Number of values: 0 or 1
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IC90

Or ICSelect, IC50, GI50, GI175, GI100, GI125, GI150

  • The ICselect value is the dose from the sigmoid instance selected as the best fit for the dose response item that corresponds to the Measure response value specified by the user. For the Inhibition Measure, typical selections are 0.5 for an IC50, 0.9 for an IC90. For the Growth Inhibition Measure, useful values are 0.5 corresponding to the GI50, 1.0 corresponding to TGI (stasis), and 1.5 corresponding to the LC50 halfway between stasis and fully cidal. For more information on Measure response values see Measures in section Analytical Methods. This value is undefined for dose response items not achieving sufficient response – a fit value of at least the specified value.
  • IC Select
  • Variable type (units): Numeric
  • Number of values: 0 or 1
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Isobologram

  • The axis of the isobolograms are normalized from 0 to 1 in linear concentration units. The labeled uM concentration on each axis is the absolute concentration for each component alone, required to achieve the isobologram effect contour level.
  • The additivity line is the diagonal so, with both agents on a normalized axis, as you remove one effective dose you have to add back the other to stay on the diagonal.
  • Variable type (units): Numeric
  • Number of values: 1
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Max CI

Max Combo Effect

Max Dose Present

  • The Max Dose present value is the maximum dose present in the dose response item.
  • Max Dose - Present
  • Variable type (units): Numeric (uM)
  • Number of values: 1
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Max Response - Fit

  • The Max Response – Fit value is the max parameter of the sigmoid instance selected as the best fit for the dose response item. For more information see Dose Response Fitting in section Analytical Methods.
  • Max Response - Fit
  • Variable type (units): Numeric
  • Number of values: 1
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Max Response - Observed

  • The Max Response – Observed value is the maximum response present in the dose response item.
  • Max Response - Observed
  • Variable type (units): Numeric
  • Number of values: 1
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Max Response - Observed - Dose

  • The Max Response – Observed – Dose value is the dose at which the Max Response – Observed value is present in the dose response item.
  • Max Response - Observed - Dose
  • Variable type (units): Numeric
  • Number of values: 1
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Max Response - Observed Error

Minimum Concentration Reported

N

Next Sample Count

Next Sample Dilution Factor

Next Sample Start

Protocol

Replicate Number

Response Area

  • The "Response Area" attribute replaces all previously labeled "Response Area [xxx]" attributes as of the 2010 Q4 release of the Chalice Analysis Engine. The new attribute provides the end user with the ability to define the concentration range for the integration as part of the analysis options for each analytical request. In addition, the system administrator can modify the default values for the concentration range.
  • Response Area
  • The concentration range is defined in terms of a lower and upper bound, between which the area between the curve and the measure null effect level will be integrated. Integration is not positively gated. The lower and upper bounds may be specified in relative (ec,ic) or absolute (uM,nM) units, and may be defined using heterogeneous units. The default values as supplied by Horizon in customer deployments are as follows:
  • Lower Bound: ec10
  • Upper Bound: 1mM
  • The default value effectively identifies potent and active compounds, but removes the bias of starting screening concentration.
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Self Cross

Size

SPE Average

SPE Average Error

SPE Excess

SPE Log Chi Squared

SPE Model

SPE Parameters

SPE Volume

SPE Volume Error

Steepness

  • The Steepness value is the steepness parameter of the sigmoid instance selected as the best fit for the dose response item. For more information see Dose Response Fitting in section Analytical Methods.
  • Steepness
  • Variable type (units): Numeric
  • Number of values: 1
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Substance Id

  • The unique identifier of the substance associated with the dose response item. The unique identifier is a composite value if the dose response item represents a fixed ratio mixture.
  • Variable type (units): Text
  • Number of values: 1
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Substance Name

  • The name of the substance (i.e., drug, biologic compound, etc.) associated with the dose response item. The substance name is a composite value if the dose response item represents a fixed ratio mixture.
  • Variable type (units): Text
  • Number of values: 1
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Sum Average

Sum Average Error

Sum Excess

Sum Log Chi Squared

Sum Model

Sum Parameters

Sum Volume

Sum Volume Error

Synergy Score

Synergy Score Error

Synergy SNR

Title