Identify new targets for drug discovery using Horizon’s siRNA screening capabilities and isogenic cell line panels
We use in-house robotics-assisted liquid handling to prepare our library assay plates and are able to manage the high-throughput screening in a rapid and efficient workflow. This same infrastructure is also valuable in secondary screening and target validation, allowing us to explore orthologous approaches to validate hits, and to simultaneously progress a large number of promising targets.
Why use our siRNA screening platform
- To identify synthetic lethal targets
- To help with target identification and validation
- To reveal resistance mechanisms
- To aid patient stratification
Summary Horizon's druggable genome siRNA library
The siRNA screening platform
- Curated library of approximately 2000 siGENOME SMARTpool reagents
- Subsets of the libraries or custom libraries are also available
- Each cell line is optimised for growth conditions and transfection efficiency
- Robotic liquid handling used to generate barcoded 384-well library plates
- Cells reverse transfected with high throughput plate processing
- Endpoint viability assessed (e.g. Hoechst staining)
- Secondary screens (target validation) which use smaller libraries can be performed in 3D or in hypoxic conditions
| siRNA screen data using an isogenic cell line pair. The hits (circled) are potential synthetic lethal hits and were subject to secondary deconvolution screening. |
