GIPZ Lentiviral shRNA Libraries

Genome-wide collections for high-throughput shRNA screening

The GIPZ lentiviral shRNA collection was designed by and built in collaboration with Cold Spring Harbor Labs. Each shRNA hairpin is cloned into the pGIPZ lentiviral vector and arrayed into 96-well plates to create a renewable RNAi resource.

GIPZ lentiviral shRNA are provided as individual human or mouse constructs, target gene sets, transfection or transduction starter kits and arrayed libraries. Positive and negative GIPZ shRNA controls are available in glycerol stock and viral particle formats.

Highlights

GIPZ Lentiviral shRNA advantages:

Designed for increased specificity, less toxicity
TurboGFP marks cells expressing shRNA
Efficient low copy knockdown- Important for pooled screens
Genome-wide human and mouse coverage
Perform efficient RNAi in primary and non-dividing cells
Guaranteed* knockdown

Please note that the pGIPZ and pTRIPZ vectors are not compatible with third-generation packaging systems, such as ViraPower™ from Invitrogen. We recommend the Trans-Lentiviral™ Packaging System for use with our vectors.

* One out of three GIPZ Lentiviral shRNA is guaranteed to produce gene knockdown by ≥ 70% at the mRNA level when used with a functioning positive control. Knockdown should be compared to non-silencing control, and experimental workflow must be controlled using the provided GIPZ GAPDH positive control.

Shipping Information

GIPZ lentiviral shRNA libraries are provided in 96-well microtiter plates containing frozen stock cultures of E.coli in LB (low salt) broth with 8% glycerol, carbenicillin (100 µg/mL), and zeocin (25 µg/mL). Individual shRNA constructs are shipped as glycerol stock cultures on wet ice. We check all cultures for growth prior to shipment. shRNA clones must be stored at -80°C.