Zoonome Custom siRNA Libraries
On-demand custom siRNA screening libraries for any species. Ensure library specificity with algorithm-designed siRNA produced with a novel enzymatic method.
Supporting large functional genomics studies in non-human or mouse models. Screen 1000 gene targets or up to the whole genome, helping you find the right solution! All that's required is species information and a gene list to get a targeted siRNA library for your model organism.
Working with alternative species (dog, pig, rat, cow, mosquito, hamster, monkey, etc.)? Utilize a novel enzymatic method to generate large arrayed libraries of algorithm-designed siRNAs for any species with an annotated genome!*Request pricing - Zoonome custom siRNA libraries »
- Novel enzymatic process for generation of functional, 21 mer siRNA duplexes for arrayed knockdown studies
- On-demand plated collections of pooled siRNAs (4 algorithm-designed siRNAs in a single well) for RNAi screening studies in non-human or mouse cells
- Minimum 1000 genes up to whole genome collections for any organism with an annotated genome
- Expert siRNA design from the SMART selection algorithm
- Provided in 384-well plates (96-well upon request) for high-throughput studies
Learn more about Zoonome siRNA libraries and pricing, submit a price quote request and have a dedicated team member contact you. Due to their highly custom nature, all Zoonome libraries are sold by quote only. Note that orders can take up to 12 weeks to fulfill, so don't delay and inquire today!
The following species have been pre-validated to have an annotated genome database in Ensembl™ (Genome Research Limited) or RefSeq that is suitable for generation of genome wide siRNA designs for creation of a Zoonome siRNA library.
Can't see species of interest on the list?
No problem. Just submit it, along with the other requested information on the Request pricing form, and we will determine feasibility for you.
|Chinese Hamster||Cricetulus griseus|
|Ferret||Mustela putorius furo|
|Golden Hamster||Mesocricetus auratus|
|Gorilla||Gorilla gorilla gorilla|
|Guinea Pig||Cavia porcellus|
|Olive Baboon||Papio anubis|
|Orangutan||Pongo pymaeus abelii|
|Rhesus Monkey||Macaca mulatta|
|Rhinoceros||Ceratotherium simum simum|
|Zebra Finch||Taeniopygia guttata|
|Nile Tilapia||Oreochromis niloticus|
|American Alligator||Alligator mississippiensis|
|Chinese Alligator||Alligator sinensis|
|Monarch Butterfly||Danaus plexippus|
|Sea Squirt||Ciona intestinalis|
|Purple Sea Urchin||Strongylocentrotus purpuratus|
Enzymatic methods for siRNA production involve more steps than chemical synthesis, but are less expensive overall and result in a smaller quantity that is appropriate for a single end-user. Zoonome siRNA libraries utilize novel enzymatic methods to generate on-demand arrayed siRNA libraries for model species where synthetic libraries are not available.
Functionality of Zoonome siRNA (z-siRNA) in CHO-K1 cells
Figure 1. CHO-K1 (Chinese hamster) cells were treated with z-siRNA pools targeting 16 genes and assessed for functional knockdown (upper graph) and cell viability (lower graph). All z-siRNA reagents achieved potent knockdown (>75%) while maintaining high cellular viability. Z-siRNAs at 25 nM were delivered with DharmaFECT 4, 0.5 ug per well and assayed 48 hr post-transfection.