RTF siRNA Libraries
Enable RNAi screening without the need for high-throughput automation. Human and mouse SMARTpool siRNA libraries in a novel predispensed format.
Reverse transfection format (RTF) siRNA libraries are provided as multiple single-use plate sets. This unique pre-plated format reduces hands-on time for faster screening results.
SMARTpool siRNA and validated control reagents are predispensed at assay-ready amounts in replicate 96-well cell culture plates. No special handling is required prior to use. Simply resuspend the siRNA and add your cells.
- Each library supplied as six replicate plate sets containing 6.25 nmol of siRNA per well; sufficient to run two screens in triplicate (additional plate sets available)
- RTF Optimization Plates are available to optimize transfection, controls, and cell density for your assay prior to beginning your screen
- RTF libraries are available in clear-bottom plates with clear, black, or white-walled wells to support assays involving fluorescent or luminescent detection methods
Click on a collection ✔ to view availability and ordering information for a RTF siRNA library.
If you have a gene list of your own not reflected in these predefined collections, submit a quote request for a custom siRNA library.
|Cell Cycle Regulation||✔||✔||✔||✔|
|G Protein-Coupled Receptors (GPCR)||✔||✔||✔||✔|
|Ubiquitin Conjugation Subset 1||✔||✔||✔||✔|
|Ubiquitin Conjugation Subset 2||✔||✔||✔||✔|
|Ubiquitin Conjugation Subset 3||✔||✔||✔||✔|
Which siRNA is right for you?
Horizon offers four complete predesigned product lines across human, mouse and rat genomes. Use the table below to assist you in determining the right siRNA product line for your needs.
|Cost-effective, efficient silencing||High specificity for reduced off-targets + efficient silencing||Highly specific knockdown of long noncoding RNA (lncRNA)||Target silencing in difficult-to-transfect cells|
|siGENOME siRNA||ON‑TARGETplus siRNA||Lincode siRNA||Accell siRNA|
|Predesigned for human, mouse and rat protein-coding genes||✔||✔||✔|
|Predesigned for human and mouse long noncoding RNA (lncRNA)||✔|
|Recommended for transfectable mammalian cells in culture||✔||✔||✔|
|Recommended for neuronal, suspension, primary and other difficult-to-transfect cells||✔|
|Recommended transfection reagent||DharmaFECT transfection reagent||DharmaFECT transfection reagent||DharmaFECT transfection reagent||None required|
|Available as SMARTpool reagent||✔||✔||✔||✔|
|Available as four individual siRNAs||✔||✔||✔||✔|
|Guaranteed knockdown by SMARTpool and 3 of 4 siRNAs||✔||✔|
|Sense strand is modified to prevent interaction with RISC and favor antisense strand uptake||Selective application when thermodynamic analysis indicates it necessary for favorable antisense RISC loading||✔||✔||✔|
|Antisense strand seed region is modified to destabilize off-target activity and enhance target specificity||✔||✔|
|Modifications to facilitate cellular uptake without separate transfection reagents||✔|
|Stabilizing modifications to prevent nuclease-mediated degradation||✔|
|Sequence information provided with purchase||✔||✔||✔||✔|