RTF siRNA Libraries

Enable RNAi screening without the need for high-throughput automation. Human and mouse SMARTpool siRNA libraries in a novel predispensed format.

Reverse transfection format (RTF) siRNA libraries are provided as multiple single-use plate sets. This unique pre-plated format reduces hands-on time for faster screening results.

SMARTpool siRNA and validated control reagents are predispensed at assay-ready amounts in replicate 96-well cell culture plates. No special handling is required prior to use. Simply resuspend the siRNA and add your cells.

Highlights:

  • Each library supplied as six replicate plate sets containing 6.25 nmol of siRNA per well; sufficient to run two screens in triplicate (additional plate sets available)
  • RTF Optimization Plates are available to optimize transfection, controls, and cell density for your assay prior to beginning your screen
  • RTF libraries are available in clear-bottom plates with clear, black, or white-walled wells to support assays involving fluorescent or luminescent detection methods

Click on a collection ✔ to view availability and ordering information for a RTF siRNA library.

If you have a gene list of your own not reflected in these predefined collections, submit a quote request for a custom siRNA library.

  Human Mouse
  ON-TARGETplus siGENOME ON-TARGETplus siGENOME
Optimization Plates
Cell Cycle Regulation
Cytokine Receptors  
Deubiquitinating Enzymes
DNA Damage    
Epigenetics
G Protein-Coupled Receptors (GPCR)
Ion Channels
Membrane Trafficking  
Nuclear Receptor  
Phosphatases
Proteases
Protein Kinases
Serine Proteases      
Transcription Factors      
Tyrosine Kinases
Ubiquitin Conjugation Subset 1
Ubiquitin Conjugation Subset 2
Ubiquitin Conjugation Subset 3

Which siRNA is right for you?

Horizon offers four complete predesigned product lines across human, mouse and rat genomes. Use the table below to assist you in determining the right siRNA product line for your needs.

  Cost-effective, efficient silencing High specificity for reduced off-targets + efficient silencing Highly specific knockdown of long noncoding RNA (lncRNA) Target silencing in difficult-to-transfect cells
  siGENOME siRNA ON‑TARGETplus siRNA Lincode siRNA Accell siRNA
Predesigned for human, mouse and rat protein-coding genes  
Predesigned for human and mouse long noncoding RNA (lncRNA)      
Recommended for transfectable mammalian cells in culture  
Recommended for neuronal, suspension, primary and other difficult-to-transfect cells      
Recommended transfection reagent DharmaFECT transfection reagent DharmaFECT transfection reagent DharmaFECT transfection reagent None required
Available as SMARTpool reagent
Available as four individual siRNAs
Guaranteed knockdown by SMARTpool and 3 of 4 siRNAs    
Sense strand is modified to prevent interaction with RISC and favor antisense strand uptake Selective application when thermodynamic analysis indicates it necessary for favorable antisense RISC loading
Antisense strand seed region is modified to destabilize off-target activity and enhance target specificity    
Modifications to facilitate cellular uptake without separate transfection reagents      
Stabilizing modifications to prevent nuclease-mediated degradation      
Sequence information provided with purchase