BLAST searches allow investigators to query the Dharmacon clone collection for cDNA and ORF clones containing specific sequences of interest. This is particularly useful when looking for novel or poorly-annotated genes, specific variants, or sequences that are conserved in different species. In order to identify clones that contain a consensus sequence of interest, please follow these instructions in order to successfully run the BLAST query:
- Begin by selecting either the nucleotide or peptide database
- Type a name for the BLAST search that you are performing
- Paste or enter a nucleotide or peptide sequence into the Sequence box
- Select the BLAST Database by choosing the species of the organism that you are querying in the drop-down menu
- Click "Submit"
Once the Result Name moves from the "Running Jobs" field to the "Completed Jobs" field, clones that contain a match to the queried sequence can be identified and added to the shopping cart.