5-Fusion Multiplex (Negative Control)
The 5-Fusion Multiplex (Negative Control) FFPE RNA Reference Standard is a highly-characterized, biologically-relevant quality control material used to assess the performance of targeted NGS, RT-PCR and RT-qPCR assays aimed at detecting gene fusions. Each FFPE section is composed of a formalin-fixed, paraffin-embedded (FFPE) cell line verified not to contain EML4-ALK, CCDC6-RET, SLC34A2-ROS1, TPM3-NTRK1 and ETV6-NTRK3 fusions.
Targeted NGS, endpoint RT-PCR and RT-qPCR platforms give researchers much greater power when profiling tumor samples. However, they present challenges surrounding sample variability, RNA extraction procedures, RNA quantification, platform bias, and potential failure to detect biomarkers.
With this product you are able to:
- Evaluate your workflow integrity from pre-analytical RNA extraction through to fusion detection
- Negative control for EML4-ALK, CCDC6-RET, SLC34A2-ROS1, TPM3-NTRK1 and ETV6-NTRK3 fusion detection panels
Genes Covered: Negative for EML4-ALK, CCDC6-RET, SLC34A2-ROS1, TPM3-NTRK1 and ETV6-NTRK3
Cosmic ID: COSF463, COSF408, COSF1272, COSF1271, COSF1197, COSF1196, COSF463, COSF408, COSF1272, COSF1271, COSF1197, COSF1196,
COSF1330, COSF1329, COSF572, COSF571
Fixation Method: 10% Formalin
Section Size: 10µm
Intended Use: For routine performance monitoring (Research Use Only)
Unit Size: 1 FFPE Section
Expiry: See all product shelf life information
Extractable RNA / Expected Yield: ≥ 100 ng RNA per section using the Maxwell RSC RNA FFPE Purification Kit from Promega
Biomarker-specific mRNA amplifiability: Absence of EML4-ALK, CCDC6-RET, SLC34A2-ROS1, TPM3-NTRK1 and ETV6-NTRK3 RNA fusion transcripts confirmed by endpoint RT-PCR
TATA-binding protein (TBP) mRNA amplifiability control: RNA expression confirmed by endpoint RT-PCR
Quantification: Qubit RNA HS kit from ThermoFisher as per the manufacturer’s instructions
We accept orders using the following methods.
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If you prefer to send us orders outside our online ordering system, please see the Contact Us page for telephone, fax, and email details in order to place an order. We recommend that you use our online system to identify the products you require and their respective “HD” product codes.
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- Product code(s) of the item(s) you wish to order (e.g. "HD123")
- Your name, email address, title and organization
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- Telephone number
- VAT Number (European Countries only)
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