Targeted NGS, endpoint RT-PCR and RT-qPCR platforms give researchers much greater power when profiling tumor samples. However, they present challenges surrounding sample variability, RNA extraction procedures, RNA quantification, platform bias, and potential failure to detect biomarkers.

With this product you are able to:

• Evaluate your workflow integrity from pre-analytical RNA extraction through to fusion detection
• Negative control for EML4-ALK, CCDC6-RET, SLC34A2-ROS1, TPM3-NTRK1 and ETV6-NTRK3 fusion detection panels

Technical Data

Genes Covered

Negative for EML4-ALK, CCDC6-RET, SLC34A2-ROS1, TPM3-NTRK1 and ETV6-NTRK3

Cosmic ID

COSF463 (EML4-ALK); COSF1272 (CCDC6-RET); COSF1197 (SLC34A2-ROS1); COSF1330 (TPM3-NTRK1); COSF572 (ETV6-NTRK3)

Fixation Method

10% Formalin

Section Size

10µm

Product Information

Intended Use

For routine performance monitoring (Research Use Only)

Unit Size

1 FFPE Section

General Information

Storage

4°C

Expiry

See all product shelf life information

Quality Control

Extractable RNA / Expected Yield

≥ 100 ng RNA per section using the Maxwell 16 LEV RNA FFPE Purification Kit from Promega

Biomarker-specific mRNA amplifiability

Absence of EML4-ALK, CCDC6-RET, SLC34A2-ROS1, TPM3-NTRK1 and ETV6-NTRK3 RNA fusion transcripts confirmed by endpoint RT-PCR

TATA-binding protein (TBP) mRNA amplifiability control

RNA expression confirmed by endpoint RT-PCR

Quantification

Qubit RNA HS kit from ThermoFisher as per the manufacturer’s instructions