5-Fusion Multiplex (Negative Control)

The 5-Fusion Multiplex (Negative Control) FFPE RNA Reference Standard is a highly-characterized, biologically-relevant quality control material used to assess the performance of targeted NGS, RT-PCR and RT-qPCR assays aimed at detecting gene fusions. Each FFPE section is composed of a formalin-fixed, paraffin-embedded (FFPE) cell line verified not to contain EML4-ALK, CCDC6-RET, SLC34A2-ROS1, TPM3-NTRK1 and ETV6-NTRK3 fusions.

Targeted NGS, endpoint RT-PCR and RT-qPCR platforms give researchers much greater power when profiling tumor samples. However, they present challenges surrounding sample variability, RNA extraction procedures, RNA quantification, platform bias, and potential failure to detect biomarkers.

With this product you are able to:

  • Evaluate your workflow integrity from pre-analytical RNA extraction through to fusion detection
  • Negative control for EML4-ALK, CCDC6-RET, SLC34A2-ROS1, TPM3-NTRK1 and ETV6-NTRK3 fusion detection panels

 

Technical Data

Genes Covered: Negative for EML4-ALK, CCDC6-RET, SLC34A2-ROS1, TPM3-NTRK1 and ETV6-NTRK3

Cosmic ID: COSF463, COSF408, COSF1272, COSF1271, COSF1197, COSF1196, COSF463, COSF408, COSF1272, COSF1271, COSF1197, COSF1196,
COSF1330, COSF1329, COSF572, COSF571

Fixation Method: 10% Formalin

Section Size: 10µm

 

Product Information

Intended Use: For routine performance monitoring (Research Use Only)

Unit Size: 1 FFPE Section

 

General Information

Storage: 4°C

Expiry: See all product shelf life information

 

Quality Control

Extractable RNA / Expected Yield: ≥ 100 ng RNA per section using the Maxwell RSC RNA FFPE Purification Kit from Promega

Biomarker-specific mRNA amplifiability: Absence of EML4-ALK, CCDC6-RET, SLC34A2-ROS1, TPM3-NTRK1 and ETV6-NTRK3 RNA fusion transcripts confirmed by endpoint RT-PCR

TATA-binding protein (TBP) mRNA amplifiability control: RNA expression confirmed by endpoint RT-PCR

Quantification: Qubit RNA HS kit from ThermoFisher as per the manufacturer’s instructions