Horizon DNA standards eliminate the variability associated with patient-derived reference standards, and avoid the hassle of sourcing, characterizing, and documenting your own cell line mixes. The standard is provided at an allelic frequency of 50%, and may be diluted using the corresponding wild-type standard to generate a lower allelic frequencies for limit of detection studies. Horizon’s Base-Seq products are all derived from human cell lines, and are high molecular weight DNA. This format ensures that they may be applied to a wide-range of assays including qPCR, Sanger sequencing, next-generation sequencing, mass array, and more.
With this product you are able to:
- Analyze the sensitivity of your assay
- Gain certainty of the limit of detection
- Optimize and validate new cancer panels and routinely monitor the performance of your assay
Synonyms: B-Raf, BRAF1
Format: Genomic DNA
DNA Base Change: GTG→GAG
NCBI Reference Assembly SNP: rs113488022
Cosmic ID: COSM476
Buffer: Tris-EDTA (10mM Tris-HCl, 1mM EDTA), pH 8.8
Intended Use: For routine performance monitoring (Research Use Only)
Unit Size: 1µg
Allelic Frequency: 50%
Cell Line Background: HCT116Exome sequencing data: Download additional information on variants present in the parental cell lines
Allelic Frequency: Droplet Digital PCR™
Genotype: Sanger sequencing of locus specific PCR
Quality: Agarose gel electrophoresis
Quantification: Spectrophotometry (A260)
Genotype: BRAF (V600E/+)
DNA was run on a 1% agarose gel and shows a single high-molecular weight band
Chromatograph showing heterozygosity for the B-Raf V600E mutation within B-Raf exon 15 (SNP accession number: rs113488022)
Please see our terms and conditions of ordering.
If you need any support, advice or additional documentation then don't hesitate to contact us here. We accept orders using the following methods:
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If you prefer to send us orders outside our online ordering system, please see the Contact Us page for telephone, fax, and email details in order to place an order. We recommend that you use our online system to identify the products you require and their respective “HD” product codes. To order offline we will require the following information:
- Product code(s) of the item(s) you wish to order (e.g. "HD123")
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- Telephone number
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Shipping information for all territories can be found on our Shipping Charges page. This includes Shipping and Handling fees, as well as shipping conditions for each product range.
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