BRAF V600E Reference Standard, 50%
The BRAF V600E Reference Standard is a highly-characterized, biologically-relevant quality control material used to assess the performance of assays that detect somatic mutations, such as Sanger and qPCR sequencing assays.
Horizon DNA standards eliminate the variability associated with patient-derived reference standards, and avoid the hassle of sourcing, characterizing, and documenting your own cell line mixes. The standard is provided at an allelic frequency of 50%, and may be diluted using the corresponding wild-type standard to generate a lower allelic frequencies for limit of detection studies. Horizon’s Base-Seq products are all derived from human cell lines, and are high molecular weight DNA. This format ensures that they may be applied to a wide-range of assays including qPCR, Sanger sequencing, next-generation sequencing, mass array, and more.
With this product you are able to:
- Analyze the sensitivity of your assay
- Gain certainty of the limit of detection
- Optimize and validate new cancer panels and routinely monitor the performance of your assay
Product Description: BRAF V600E Reference Standard, 50%
Catalog number: HD238
Amino Acid Change: V600E
Nucleotide change: c.1799T>A
Variant Allele Frequency: 50%
Cosmic Genomic Mutation ID: COSV56056643
Cosmic Legacy ID: COSM476
GRCh38 position: 7:140753336
Format: Genomic DNA
DNA Base Change: GTG→GAG
NCBI Reference Assembly SNP: rs113488022
Cosmic ID: COSM476
Buffer: Tris-EDTA (10mM Tris-HCl, 1mM EDTA), pH 8.8
Intended Use: For routine performance monitoring (Research Use Only)
Unit Size: 1µg
Allelic Frequency: 50%
Expiry: See all product shelf life information
Cell Line Background: HCT116Exome sequencing data: Download additional information on variants present in the parental cell lines
Allelic Frequency: Droplet Digital PCR™
Genotype: Sanger sequencing of locus specific PCR
Quality: Agarose gel electrophoresis
Quantification: Spectrophotometry (A260)
Genotype: BRAF (V600E/+)
DNA was run on a 1% agarose gel and shows a single high-molecular weight band
Chromatograph showing heterozygosity for the B-Raf V600E mutation within B-Raf exon 15 (SNP accession number: rs113488022)
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