Horizon FFPE Reference standards are cell-line derived making them reproducible, renewable, and affordable experimental controls for molecular diagnostic assays. They are formulated to mimic patient samples and serve as excellent quality controls by acting as performance indicators for the whole diagnostic workflow.
Horizon FFPE standards eliminate the variability associated with patient-derived reference standards, and avoid the hassle of sourcing, characterizing, and documenting your own cell line mixes. Using a proprietary method of mild fixation and homogenous paraffin embedding, we generate highly reproducible and consistent FFPE curls. Our standards yield high molecular weight DNA which can be analyzed in a wide-range of assays including qPCR, Sanger sequencing and next-generation sequencing.
Product Description: EGFR L858R Reference Standard, FFPE, 5%
Catalog number: HD129
Amino Acid Change: L858R
Nucleotide change: c.2573T>G
Variant Allele Frequency: 5%
Cosmic Genomic Mutation ID: COSV51765161
Cosmic Legacy ID: COSM6224
GRCh38 position: 7:55191822
Unit Size: 15 µm section
Fixation Method: 4% PFA (w/v)
Expected DNA Yield: ≥ 400ng Using Maxwell RSC Plus FFPE DNA Extraction kit
Cell Line Background: RKO
Exome Sequencing Data: Download additional sequencing data from the parental cell
Genotype and Allelic Frequency: Droplet Digital PCRTM
DNA integrity: Agarose gel electrophoresis
Manufacturing Quality: ISO 13485:2016
Use: Research Use Only
Cell Line Characterization
Chromatograph showing heterozygosity for the EGFR L858R within EGFR exon 21 (SNP accession number: rs121434568)
We accept orders using the following methods.
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- Product code(s) of the item(s) you wish to order (e.g. "HD123")
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