Horizon FFPE standards eliminate the variability associated with patient-derived reference standards, and avoid the hassle of sourcing, characterizing, and documenting your own cell line mixes. Using a proprietary method of mild fixation and homogenous paraffin embedding, we generate highly-reproducible and consistent FFPE curls. With any commercially available FFPE extraction protocol, our standards yield high molecular weight DNA. This format ensures that they may applied to a wide-range of assays including qPCR, Sanger sequencing, next-generation sequencing, mass array, and more.

With this product you are able to:

• Evaluate of workflow integrity from pre-analytical DNA extraction to post-analytical bioinformatics
• Analyze the sensitivity of your workflow
• Optimize and validate new cancer panels and routinely monitor the performance of your assay

Product Information

Product Description: KRAS Wild Type Reference Standard

Catalog Number: HD135

Synonyms: C-Ki-Ras, K-RAS2B, KI-RAS, KRAS2, RASK2

Format: FFPE

Fixation Method: 4% PFA (w/v)

Section Size: 15µm

Cell Density: 3 x 10⁸ cells per block. Approx. 3.5 x 10⁵ cells per section

Expected DNA Yield: ≥ 400 ng using Promega Maxwell RSC Plus Extraction kit

Intended Use: Research Use Only

Unit Size: 1 FFPE Section per vial

Extractable DNA: ≥ 400 ng per section using Promega Maxwell RSC Plus FFPE DNA Extraction kit

General Information

Allelic Frequency: WT

Storage: 4˚C

Shipping: Ambient

Expiry: See all product shelf life information

Cell Line Background: SW48

Exome sequencing data: Download additional information on variants present in the parental cell lines

Quality Control

Genotype and Allelic Frequency: Droplet Digital PCR™

DNA Integrity: Agarose gel electrophoresis

Quantification: Quantifluor™

Manufacturing Quality: ISO 13485:2016

Genotype: KRAS (+/+)

Cell Line Characterization

Sanger sequencing of locus specific PCR

Chromatogram:

Chromatograph showing heterozygosity for the KRAS A146T mutation and KRAS A146 wild type

Primers: