Horizon FFPE Reference standards are cell-line derived making them reproducible, renewable, and affordable experimental controls for molecular diagnostic assays. They are formulated to mimic patient samples and serve as excellent quality controls by acting as performance indicators for the whole diagnostic workflow.

Technical Information

Horizon FFPE standards eliminate the variability associated with patient-derived reference standards, and avoid the hassle of sourcing, characterizing, and documenting your own cell line mixes. Using a proprietary method of mild fixation and homogenous paraffin embedding, we generate highly reproducible and consistent FFPE curls. Our standards yield high molecular weight DNA which can be analyzed in a wide-range of assays including qPCR, Sanger sequencing and next-generation sequencing.

Product Information

Synonyms: PI3Kα

Format: FFPE

Fixation Method: 4% PFA (w/v)

Section Size: 15µm

Cell Density: 3 x 10⁸ cells per block. Approx. 3.5 x 10⁵ cells per section

Expected DNA Yield: ≥ 400 ng using Promega Maxwell RSC Plus Extraction kit

Intended Use: Research Use Only

Unit Size: 1 FFPE Section per vial

Extractable DNA: ≥ 400 ng per section using Promega Maxwell RSC Plus FFPE DNA Extraction kit

General Information

Allelic Frequency: WT

Storage: 4˚C

Expiry: See all product shelf life information

Cell Line Background: SW48

Exome sequencing data: Download additional information on variants present in the parental cell lines

Quality Control

Genotype and Allelic Frequency: Droplet Digital PCRTM

DNA integrity: Agarose gel electrophoresis

Quantification: Quantifluor™

Manufacturing Quality: ISO 13485:2016

Use: Research Use Only

Cell Line Characterization

Chromatogram:

Chromatograph showing heterozygosity for the E545K mutation and PIK3CA; wild type

Primers:

PI3Kα E545

For Sanger sequencing of PI3Kα E545 we use the following primer set:

PI3Kα h2047

For Sanger sequencing of PI3Kα h2047 we use the following primer set: