5 steps to a stable Cas9-expressing cell line

It’s easy with Edit-R™ CRISPR-Cas9 products!

Using Edit-R Lentiviral Cas9 nuclease particles, you can generate cell lines stably expressing Cas9 nuclease in only a few steps.

Step 1:

  • Plate cells in a 24-well plate in growth medium and incubate overnight

Step 2:

  • Thaw the Edit-R Lentiviral Cas9 particles and mix them into transduction medium

Step 3:

  • Add the transduction medium containing the lentiviral particles to your plated cells and incubate

Step 4:

  • 24-48 hours post-transduction, begin antibiotic selection by replacing the medium with medium containing blasticidin

Step 5:

  • Once the antibiotic selection is complete and cells are growing normally, expand them and freeze a sufficient number of aliquots for your downstream gene editing experiments

You can choose to perform clonal isolation and characterization, or simply utilize the mixed population Cas9 cell line for gene knockout screening with Edit-R Lentiviral sgRNA Pooled Libraries or arrayed Edit-R crRNA Libraries.

If you need control over the timing of Cas9 expression, or are concerned about cellular changes from constitutive expression, try Edit-R Inducible Lentiviral Cas9 Nuclease for straightforward on/off control with the Tet-on 3G system.

View the complete technical manual to learn more »

Additional Resources

Inducible Cas9 Nuclease - Application

  • Get an overview on how to control the timing of gene editing events

CRISPR-Cas9 Gene Editing - Products

  • Optimized tools for high-confidence genome engineering

CRISPR-Cas9 Gene Editing - Applications

  • CRISPR-Cas9 systems can be used with custom RNA guides for several gene editing applications

CRISPR-Cas9 Gene Editing - Resources

  • Find product guides, FAQs and more.