CRISPR-Cas9 has changed the landscape of gene modulation research
Cas9 expression is the key to higher gene editing efficiency!
As genome engineering has taken hold in the world of gene modulation research, it has become common knowledge that expression of Cas9 nuclease is the rate limiting step. There are multiple methods by which you can express Cas9 in your cells of interest ranging from constitutive expression by genome integration to a very transient method such as directly introducing the protein to the system. The choice of which method is right for your research depends on experimental design and desired results outcome.
We have put together an informative infographic regarding Cas9 which includes delivery, timelines, and fun facts which would look great on the wall in your lab or office.