This video describes the use of DharmaFECT Duo transfection reagent to deliver both RNA and DNA in the same transfection. Although applicable to many experimental scenarios, the co-delivery of crRNA and tracrRNA along with a Cas9 expressing DNA plasmid (Edit-R Gene Editing Platform) is used in this example protocol. An RNAi rescue experiment is another example of an application where efficient delivery of both RNA and DNA in the same transfection is desirable.

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