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This webinar will describe the level of knockdown that can be achieved with degron-tagged CRISPR interference proteins, how best to deploy dox-inducible Cas9, and various approaches to delete wild-type alleles of genes so as to replace them with inactive alleles that could be applied at scale.
What You Will Learn
- How many of the hits from primary CRISPR screens are validated in follow up work
- How difficult target validation really is
- The qualitatively different nature of results bearing in target essentiality that one gets with tool compounds and CRISPR/Cas9