This webinar will describe the level of knockdown that can be achieved with degron-tagged CRISPR interference proteins, how best to deploy dox-inducible Cas9, and various approaches to delete wild-type alleles of genes so as to replace them with inactive alleles that could be applied at scale.

What You Will Learn

  • How many of the hits from primary CRISPR screens are validated in follow up work
  • How difficult target validation really is
  • The qualitatively different nature of results bearing in target essentiality that one gets with tool compounds and CRISPR/Cas9