This is an introduction to the biological mechanism of RNA interference (RNAi) and experimental application of short, interfering RNA (siRNA). The key steps of the endogenous RNAi pathway are explained to provide context for the mechanism of targeted gene knockdown with siRNA. The critical factors that affect siRNA function and specificity (off-targeting) are covered in detail. The best-practices for practical application of siRNA experimental design is discussed. This includes proper use of positive and negative controls, optimization of transfection and siRNA concentration. Measurement of gene knockdown and appropriate interpretation of experimental outcomes round out the discussion. In this webinar, you will learn:
- Key steps of the endogenous RNAi pathway
- siRNA mechanism of action
- Best practices for siRNA experimental design
- Appropriate interpretation of experimental outcomes
Sr. Product Manager
Louise Baskin is a Senior Product Manager at Dharmacon, having joined the company in 2005. She holds responsibility for all synthetic RNA products, including custom and predesigned siRNA and microRNA reagents. She has led the development and commercial launch of multiple genome-wide product lines, including specificity-enhanced siRNA, self-delivering siRNA, and products for silencing long noncoding RNA.