This is an introduction to the biological mechanism of RNA interference (RNAi) and experimental application of short, interfering RNA (siRNA). The key steps of the endogenous RNAi pathway are explained to provide context for the mechanism of targeted gene knockdown with siRNA. The critical factors that affect siRNA function and specificity (off-targeting) are covered in detail. The best-practices for practical application of siRNA experimental design is discussed. This includes proper use of positive and negative controls, optimization of transfection and siRNA concentration. Measurement of gene knockdown and appropriate interpretation of experimental outcomes round out the discussion. In this webinar, you will learn:
- Key steps of the endogenous RNAi pathway
- siRNA mechanism of action
- Best practices for siRNA experimental design
- Appropriate interpretation of experimental outcomes
Louise studied biology at Creighton University and received her Master of Science degree in Molecular Biology and Genetics from Northwestern University.