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Webinar: How to use CRISPR-Cas9 for knockout, knock-in, and gene activation

The rapid adoption and ease of use of CRISPR-Cas9 has driven the development of applications for gene knockout, precise gene editing, and transcriptional regulation. This allows researchers to more quickly define the role of their gene in various biological contexts to progress the understanding of human disease. The wide availability of genome engineering tools makes these applications highly accessible, but still requires careful consideration for reagent selection, design, and experimental optimization. In this webinar, we will demonstrate applications of the CRISPR-Cas9 system for creation of a knockout cell line, precise knock-in, and activation of gene transcription.

Learning Objectives:

  • Learn how to successfully generate and characterize knockout cell lines
  • Recognize the key factors for successful fluorescent knock-in, as demonstrated in K562 and iPS cell lines
  • Understand how to use adapted CRISPR-Cas9 reagents for robust activation of one or more genes for gain-of-function studies
  • Discover how our design tools, reagents, and services can help with your knockout, knock-in, or gene activation experiments

Featured Resources

CRISPR-Cas9 Gene Editing - Products

  • Optimized tools for high-confidence genome engineering

CRISPR-Cas9 Guide RNA Functionality

  • Development of an algorithm for functional knockout, not just cutting

Synthetic single guide RNA for CRISPR-Cas9 experiments

  • CRISPR-Cas9 genome editing with a synthetic single guide RNA (sgRNA)

Homology-directed repair (HDR) with a plasmid donor

  • Considerations for successful HDR experimental design

CRISPRa transcriptional activation

  • Background and experimental considerations for CRISPRa