CDC assay quote request

Compound screening by complement activation on cell lines

When time is crucial in developing new drugs, take advantage of the semi-automation technologies offered in our standard assay services.
From planning and execution to data analysis, we have the expertise to move your project forward.
One of the team will be in touch within 48 hours

 

Horizon's standard CDC assay services

Pick and choose cells from the OncoSignature™ panel during a consultation with our scientists, who can help guide you to the cell panel that best suits your needs.

Standard base project: 

  • 2 compounds per cell line (control IgG included)
  • Rituximab in Raji cells as an assay control

Standard CDC assay read-outs 

  • Part 1: percentage of calcein retention per cell line
  • Part 2: percentage cell lysis by fluorescence of released calcein per compound/cell line
    •  Assay run in quadruplicate
    • 9 point dose response (8 dilutions plus vehicle)
    • 3 or 4-fold dilution range

Our cloud-based data report includes:

  • Cell quality control metrics
  • Assay performance QC - Raji + Rituximab
  • Results summary
    •  Cell growth characteristics 
    • Calcein retention assessment
    • CDC assay
  • EC50 values and annotated readout
  • Raw Data and Metadata

 

Service description Number of suitable compounds Catalog Number
Calcein release assessment  Up to 5 cell lines IMM02-01
Standard CDC assay - Base plate  2 compounds per one cell line IMM02-02
Standard CDC assay - Additional plate  Up to 20 compounds in up to 5 cell lines IMM02-03

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Immunology services

Answer questions in biologically relevant primary immune cell types
  • Leverage our expertise screening in immune cell types
  • Receive robust data from high-throughput assays

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