Human Duet siRNA Library - Druggable Genome
Duet siRNA Libraries are paired libraries of siGENOME and ON-TARGETplus siRNA SMARTpool reagents. The Human Duet Druggable Genome Library contains the genes that are considered potential targets for therapeutics and is delivered as nine distinct subset siRNA libraries in the 96-well format (see Figure 1, Supporting Data tab).
Features
In the 384-well format, the first eight subsets are plated continuously to reduce partial plates:
- GPCR
- Protein Kinases
- Ion Channels
- Phosphatases
- Drug Targets*
- Proteases
- Ubiquitin Conjugation 1 - Cullins, E1, E2, HECT E3 Ligases
- Ubiquitin Conjugation 2 - F-box, SOCS box E3 Ligases
- Ubiquitin Conjugation 3 - RING finger and RING finger-like E3 Ligases
*Includes genes involved with apoptosis, senesence, nucleic acid binding, autophagy, DNA repair, and characterized nuclear receptors.
Highlights
- Increase confidence in your RNAi screen with two guaranteed, highly-functional SMARTpool siRNA reagents (see Figure 2, Supporting Data tab)
- Screen the libraries side-by-side to move high confidence hits through the pipeline faster (see Figure 3, Supporting Data tab)
- Use the libraries separately as a cost-effective way to obtain two guaranteed collections of highly potent siRNA reagents
siRNA Designs
Both siGENOME and ON-TARGETplus siRNA reagents utilize the same SMARTselection foundational elements for highly functional designs. ON-TARGETplus designs are additionally filtered for specificity-enhancing features that improve the potential for unique designs.
- In 30% of genes (across the human genome), all eight siRNA sequences targeting a single gene are unique.
- Only 5% of genes share four siRNA sequences across both product lines.
- Even in cases of identical designs, it has been widely demonstrated that ON-TARGETplus specificity-enhancing modifications provide distinct and reduced off-target signatures and thus can be treated as unique silencing reagents.
