Human ON-TARGETplus RTF - Epigenetics
Ideal for labs who want to carry out siRNA screens but lack high-throughput capabilities.
The ON-TARGETplus Human Epigenetics siRNA Library targets enzyme classes characterized to play a role in epigenetic mechanisms (see Reference 1). These inheritable factors that regulate genetic expression are of growing importance to scientific understanding of disease progression and trait inheritance. Modification of histones, such as methylation or acetylation, plays a key role in epigenetics as the extent to which DNA is wrapped around these protein complexes will alter the availability of genes in those DNA segments to be activated. The Epigenetics library targets genes which encode histone and DNA modification factors like methyltransferases, acetyltransferases, kinases and deacetylases, in addition to genes with a role in chromatin remodeling and histone ubiquitination.
RTF siRNA libraries are provided as multiple single-use plate sets – just rehydrate and add cells. This unique pre-plated format reduces hands-on time for faster screening results.
ON-TARGETplus modifications combine sense strand inactivation with a novel seed-region modification. This patented siRNA approach is the best strategy to prevent off-target effects caused by both the sense and antisense strands while maintaining high silencing potency.
Highlights
- Six ready-to-use 96-well plate sets provided for two triplicate screens
- Pre-plated, validated RNAi controls included
- No aliquoting necessary, just resuspend and add cells
- siRNA reagents provided in clear plates at 6.25 pmol per well (50 nM final screening concentration)
- Black or white clear-bottom plates available to support assays involving fluorescent or luminescent detection
| Well | Pre-plated control | Control Catalog No. |
|---|---|---|
| A1 | ON-TARGETplus Non-targeting siRNA #1 | D-001810-01 |
| B1 | ON-TARGETplus Non-targeting siRNA #2 | D-001810-02 |
| C1 | ON-TARGETplus Non-targeting siRNA #3 | D-001810-03 |
| D1 | ON-TARGETplus Non-targeting siRNA #4 | D-001810-04 |
| E1 | ON-TARGETplus Non-targeting pool | D-001810-10 |
| F1 | ON-TARGETplus Human Cyclophilin B control pool | D-001820-10 |
Experimental considerations
DharmaFECT transfection reagents are highly recommended for use with RTF libraries and should be purchased separately. Refer to the DharmaFECT Cell Type Guide to find the appropriate formulation for your cell type.
Gene Targets
For a complete list of target genes in this siRNA Library, please contact Technical Support or your local Sales Representative.
