Human ON-TARGETplus RTF - Phosphatases
Ideal for labs who want to carry out siRNA screens but lack high-throughput capabilities
With the Human ON-TARGETplus RTF Phosphatases siRNA library, researchers receive siRNAs targeting enzymes with known or predicted phosphatase activity. Phosphatases play key roles in many biological processes including cell-cycle progression, replication, transcription, and RNA processing. Cells depend on phosphatases to maintain tight control over the activity of several metabolic enzymes and signal transducing factors.
RTF siRNA libraries are provided as multiple single-use plate sets – just rehydrate and add cells. This unique pre-plated format reduces hands-on time for faster screening results.
ON-TARGETplus modifications combine sense strand inactivation with a novel seed-region modification. This patented siRNA approach is the best strategy to prevent off-target effects caused by both the sense and antisense strands while maintaining high silencing potency.
Highlights
- Six ready-to-use 96-well plate sets provided for two triplicate screens
- Pre-plated, validated RNAi controls included
- No aliquoting necessary, just resuspend and add cells
- siRNA reagents provided in clear plates at 6.25 pmol per well (50 nM final screening concentration)
- Black or white clear-bottom plates available to support assays involving fluorescent or luminescent detection
| Well | Pre-plated control | Control Catalog No. |
|---|---|---|
| A1 | ON-TARGETplus Non-targeting siRNA #1 | D-001810-01 |
| B1 | ON-TARGETplus Non-targeting siRNA #2 | D-001810-02 |
| C1 | ON-TARGETplus Non-targeting siRNA #3 | D-001810-03 |
| D1 | ON-TARGETplus Non-targeting siRNA #4 | D-001810-04 |
| E1 | ON-TARGETplus Non-targeting pool | D-001810-10 |
| F1 | ON-TARGETplus Human Cyclophilin B control pool | D-001820-10 |
Experimental considerations
DharmaFECT transfection reagents are highly recommended for use with RTF libraries and should be purchased separately. Refer to the DharmaFECT Cell Type Guide to find the appropriate formulation for your cell type.
Gene Targets
For a complete list of target genes in this siRNA Library, please contact Technical Support or your local Sales Representative.
