Human ON-TARGETplus RTF - Protein Kinase

Screening services
Dharmacon Screening libraries
Human ON-TARGETplus RTF - Protein Kinase

Human ON-TARGETplus RTF - Protein Kinase

Ideal for labs who want to carry out siRNA screens but lack high-throughput capabilities

Ready-to-use arrayed siRNA library in single-use plate sets. Just rehydrate and add cells for RNAi screening of human kinases. Optimization plates available.

The Human ON-TARGETplus RTF Protein Kinase siRNA Library includes siRNA reagents directed against phylogenetically related kinases. Protein kinases are key regulators of cell function and are known to direct the activity, localization, and function of many proteins. They are prominent in signal transduction and are crucial for the coordination of complex cellular functions such as cell cycle regulation, gene transcription, and apoptosis.

RTF siRNA libraries are provided as multiple single-use plate sets – just rehydrate and add cells. This unique pre-plated format reduces hands-on time for faster screening results.

ON-TARGETplus modifications combine sense strand inactivation with a novel seed-region modification. This patented siRNA approach is the best strategy to prevent off-target effects caused by both the sense and antisense strands while maintaining high silencing potency.

Highlights

Six ready-to-use 96-well plate sets provided for two triplicate screens
Pre-plated, validated RNAi controls included
No aliquoting necessary, just resuspend and add cells
siRNA reagents provided in clear plates at 6.25 pmol per well (50 nM final screening concentration)
Black or white clear-bottom plates available to support assays involving fluorescent or luminescent detection

For a diagram of the RTF Library Plate layout, see Figure 1 on the Supporting Data tab.
Well	Pre-plated control	Control Catalog No.
A1	ON-TARGETplus Non-targeting siRNA #1	D-001810-01
B1	ON-TARGETplus Non-targeting siRNA #2	D-001810-02
C1	ON-TARGETplus Non-targeting siRNA #3	D-001810-03
D1	ON-TARGETplus Non-targeting siRNA #4	D-001810-04
E1	ON-TARGETplus Non-targeting pool	D-001810-10
F1	ON-TARGETplus Human Cyclophilin B control pool	D-001820-10

Experimental considerations

DharmaFECT transfection reagents are highly recommended for use with RTF libraries and should be purchased separately. Refer to the DharmaFECT Cell Type Guide to find the appropriate formulation for your cell type.

Gene Targets

For a complete list of target genes in this siRNA Library, please contact Technical Support or your local Sales Representative.