Human ON-TARGETplus RTF - Tyrosine Kinases
The only ready-to-use siRNA screening libraries available
Human ON-TARGETplus RTF Tyrosine Kinase siRNA Library includes siRNA reagents targeting tyrosine kinases; a large family of evolutionarily conserved enzymes that activate and deactivate a number of different metabolic pathways by catalyzing the phosphorylation of specific tyrosine residues on key effector proteins. Tyrosine kinases are typically classified into membrane-bound or non-membrane-bound groups and are found in nearly every mammalian cell type.
RTF siRNA libraries are provided as multiple single-use plate sets just rehydrate, and add cells. The pre-plated format reduces hands-on time for faster screening results.
ON-TARGETplus modifications combine sense-strand inactivation with a novel seed-region modification. This patented siRNA approach is the best strategy to prevent off-target effects caused by both the sense and antisense strands while maintaining high-silencing potency.
Highlights
- Six ready-to-use 96-well plate sets provided for two triplicate screens
- Pre-plated, validated RNAi controls included
- No aliquoting necessary, just resuspend and add cells
- siRNA reagents provided in clear plates at 6.25 pmol per well (50nM final screening concentration)
- Black or white clear-bottom plates available to support assays involving fluorescent or luminescent detection
| Well | Pre-plated controls in ON-TARGETplus RTF Libraries | |
| A1 | ON-TARGETplus Non-targeting siRNA #1 | D-001810-01 |
| B1 | ON-TARGETplus Non-targeting siRNA #2 | D-001810-02 |
| C1 | ON-TARGETplus Non-targeting siRNA #3 | D-001810-03 |
| D1 | ON-TARGETplus Non-targeting siRNA #4 | D-001810-04 |
| E1 | ON-TARGETplus Non-targeting pool | D-001810-10 |
| 1F | ON-TARGETplus Human Cyclophilin B control pool | D-001820-10 |
For a diagram of the RTF Library Plate layout, see Figure 1 on the Supporting Data tab.
Experimental considerations
DharmaFECT transfection reagents are highly recommended for use with RTF libraries and should be purchased separately. Refer to the DharmaFECT Cell Type Guide to find the appropriate formulation for your cell type.
Gene Targets
For a complete list of target genes in this siRNA Library, please contact Technical Support.
