Human ON-TARGETplus RTF - Ubiquitin Conjugation Subset 3
Ideal for labs who want to carry out siRNA screens but lack high-throughput capabilities
The Human ON-TARGETplus RTF Ubiquitin Conjugation 3 siRNA Library contains the majority of known ubiquitin ligases. RING finger and most RING finger-like E3 ligases form a site of interaction with E2s through a platform generated as a consequence of coordination of two zinc ions. A20-like proteins, also included in this set, contain a single coordination site for zinc. This set contains siRNA for well-known E3s such as BRCA1, MDM2 and CBL family members.
ON-TARGETplus modifications combine sense strand inactivation with a novel seed-region modification. This patented siRNA approach is the best strategy to prevent off-target effects caused by both the sense and antisense strands while maintaining high silencing potency.
Highlights
- Six ready-to-use 96-well plate sets provided for two triplicate screens
- Pre-plated, validated RNAi controls included
- No aliquoting necessary, just resuspend and add cells
- siRNA reagents provided in clear plates at 6.25 pmol per well (50 nM final screening concentration)
- Black or white clear-bottom plates available to support assays involving fluorescent or luminescent detection
| Well | Pre-plated control | Control Catalog No. |
|---|---|---|
| A1 | ON-TARGETplus Non-targeting siRNA #1 | D-001810-01 |
| B1 | ON-TARGETplus Non-targeting siRNA #2 | D-001810-02 |
| C1 | ON-TARGETplus Non-targeting siRNA #3 | D-001810-03 |
| D1 | ON-TARGETplus Non-targeting siRNA #4 | D-001810-04 |
| E1 | ON-TARGETplus Non-targeting pool | D-001810-10 |
| F1 | ON-TARGETplus Human Cyclophilin B control pool | D-001820-10 |
Experimental considerations
DharmaFECT transfection reagents are highly recommended for use with RTF libraries and should be purchased separately. Refer to the DharmaFECT Cell Type Guide to find the appropriate formulation for your cell type.
Gene Targets
For a complete list of target genes in this siRNA Library, please contact Technical Support or your local Sales Representative.
For a thorough investigation of the ubiquitin pathway, you may also consider these additional siRNA libraries:
