Mouse ON-TARGETplus RTF - Nuclear Receptors
The only ready-to-use siRNA screening libraries available
The Mouse ON-TARGETplus RTF Nuclear Receptor siRNA Library enables in-depth study of distinct nuclear receptors, including estrogen receptor 1 and 2 (ESR1 and ESR2), retinoic acid receptor alpha (RARa), and vitamin D (1,25-dihydroxyvitamin D3) receptor (VDR). Nuclear receptors form a large family of ligand-activated transcription factors that control many aspects of cellular metabolism including lipid and glucose homeostasis, growth and differentiation, and inflammation.
RTF siRNA libraries are provided as multiple single-use plate sets - just rehydrate and add cells. This unique pre-plated format reduces hands-on time for faster screening results.
ON-TARGETplus modifications combine sense strand inactivation with a novel seed-region modification. This patented siRNA approach is the best strategy to prevent off-target effects caused by both the sense and antisense strands while maintaining high silencing potency.
Highlights
- Six ready-to-use 96-well plate sets provided for two triplicate screens
- Pre-plated, validated RNAi controls included
- No aliquoting necessary - just resuspend, and add cells
- siRNA reagents provided in clear plates at 6.25 pmol per well (50 nM final screening concentration)
- Black or white clear-bottom plates available to support assays involving fluorescent or luminescent detection
| Well | Pre-plated control | Control Catalog No. |
|---|---|---|
| A1 | ON-TARGETplus Non-targeting siRNA #1 | D-001810-01 |
| B1 | ON-TARGETplus Non-targeting siRNA #2 | D-001810-02 |
| C1 | ON-TARGETplus Non-targeting siRNA #3 | D-001810-03 |
| D1 | ON-TARGETplus Non-targeting siRNA #4 | D-001810-04 |
| E1 | ON-TARGETplus Non-targeting pool | D-001810-10 |
| F1 | ON-TARGETplus Mouse Cyclophilin B control pool | D-001820-20 |
Experimental considerations
DharmaFECT transfection reagents are highly recommended for use with RTF libraries and should be purchased separately. Refer to the DharmaFECT Cell Type Guide to find the appropriate formulation for your cell type.
Gene Targets
For a complete list of target genes in this siRNA Library, please contact Technical Support.
