The Mouse ON-TARGETplus RTF Ubiquitin Conjugation Subset 1 siRNA library contains siRNA reagents targeting Cullin, E1, E2, and HECT E3 Ligases. Assessment of effects of E1 and E2 enzymes will provide a reasonable idea as to whether components of the ubiquitin system are involved. Also included are members of the HECT family of E3 ligases, which differ from the large majority of known E3s (RING finger and RING finger-like) in functioning as catalytic intermediates in ubiquitin conjugation.

RTF siRNA libraries are provided as multiple single-use plate sets – just rehydrate, and add cells. This unique pre-plated format reduces hands-on time for faster screening results.

ON-TARGETplus modifications combine sense strand inactivation with a novel seed-region modification. This patented siRNA approach is the best strategy to prevent off-target effects caused by both the sense and antisense strands while maintaining high-silencing potency.

Highlights

• Six ready-to-use 96-well plate sets provided for two triplicate screens
• Pre-plated, validated RNAi controls included
• No aliquoting necessary, just resuspend and add cells
• siRNA reagents provided in clear plates at 6.25 pmol per well (50 nM final screening concentration)
• Black or white clear-bottom plates available to support assays involving fluorescent or luminescent detection

Experimental considerations

DharmaFECT transfection reagents are highly recommended for use with RTF libraries and should be purchased separately. Refer to the DharmaFECT Cell Type Guide to find the appropriate formulation for your cell type.

Gene Targets

For a complete list of target genes in this siRNA Library, please contact Technical Support.

For a thorough investigation of the ubiquitin pathway, you may also consider these additional libraries:

• Deubiquitinating enzymes
• Ubiquitin Conjugation Subset 2 - F-box, SOCS box E3 Ligases
• Ubiquitin Conjugation Subset 3 - RING finger and RING finger-like E3 Ligases