Mouse RTF Optimization plates - ON-TARGETplus
Optimize conditions and select controls for your RTF screen
Three 96-well plates with pre-plated controls in Reverse Transfection Format (RTF) for optimization of conditions for your RNAi screen with RTF Mouse siRNA Libraries.
Optimization is essential for any RNAi screen. The ON-TARGETplus Optimization Plates are ideal for selection of the best transfection conditions and controls for screens in mouse cells using RTF siRNA Libraries. The plate layout enables the testing of cell density and transfection reagent amount on the same plate while also identifying the best non-targeting siRNA control for your assay.
Highlights
- Three ready-to-use replicate 96-well plates; 6.25 pmol siRNA per well (50 nM final concentration)
- Provided in clear or clear-bottomed white or black-walled plates to support assays involving fluorescent or luminescent detection
- Positive control pool targets mouse cyclophilin B (Accession NM_011149)
- Four non-targeting siRNAs and a non-targeting pool with ON-TARGETplus modifications to minimize off-target effects
| Row | Pre-plated control | Control Catalog No. |
|---|---|---|
| A | ON-TARGETplus Non-targeting siRNA #1 | D-001810-01 |
| B | ON-TARGETplus Non-targeting siRNA #2 | D-001810-02 |
| C | ON-TARGETplus Non-targeting siRNA #3 | D-001810-03 |
| D | ON-TARGETplus Non-targeting siRNA #4 | D-001810-04 |
| E | ON-TARGETplus Non-targeting pool | D-001810-10 |
| F | ON-TARGETplus Mouse Cyclophilin B control pool | D-001820-20 |
Experimental considerations
DharmaFECT transfection reagents are highly recommended for use with RTF libraries and should be purchased separately. Refer to the DharmaFECT Cell Type Guide to find the appropriate formulation for your cell type.
