Mouse siGENOME RTF - Phosphatases

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Dharmacon Screening libraries
Mouse siGENOME RTF - Phosphatases

Mouse siGENOME RTF - Phosphatases

The only ready-to-use siRNA screening libraries available

A ready-to-use reverse transfection format RNAi screening library targeting mouse phosphatases. Just resuspend pre-dispensed siRNA, and add cells. Optimization plates available.

With the Mouse siGENOME RTF Phosphatases siRNA library, researchers receive siRNAs targeting enzymes with known or predicted phosphatase activity. Phosphatases play key roles in many biological processes, including cell-cycle progression, replication, transcription, and RNA processing. Cells depend on phosphatases to maintain tight control over the activity of several metabolic enzymes and signal transducing factors.

RTF siRNA libraries are provided as multiple single-use plate sets – just rehydrate, and add cells. This unique pre-plated format reduces hands-on time for faster screening results.

siGENOME siRNAs are designed with the proprietary SMARTselection design algorithm for high-efficiency, guaranteed silencing. They also incorporate rational strand bias with application of ON-TARGET modifications to optimize antisense strand loading into RISC for effective target knockdown.

Highlights

Six ready-to-use 96-well plate sets provided for two triplicate screens
Pre-plated, validated RNAi controls included
No aliquoting necessary, just resuspend and add cells
siRNA reagents provided in clear plates at 6.25 pmol per well (50 nM final screening concentration)
Black or white clear-bottom plates available to support assays involving fluorescent or luminescent detection

For a diagram of the RTF Library Plate layout, see Figure 1 on the Supporting Data tab.
Well	Pre-plated control	Control Catalog No.
A1	siGENOME Non-targeting siRNA #2	D-001210-02
B1	siGENOME Non-targeting siRNA #3	D-001210-03
C1	siGENOME Non-targeting siRNA #4	D-001210-04
D1	siGENOME Non-targeting siRNA #5	D-001210-05
E1	siGENOME Non-targeting pool #2	D-001206-14
F1	siGENOME Cyclophilin B control pool (H, M, R) Control siRNA	D-001136-01

Experimental considerations

DharmaFECT transfection reagents are highly recommended for use with RTF libraries and should be purchased separately. Refer to the DharmaFECT Cell Type Guide to find the appropriate formulation for your cell type.

Gene Targets

For a complete list of target genes in this siRNA Library, please contact Technical Support or your local Sales Representative.