Mouse siGENOME RTF - Ubiquitin Conjugation Subset 1
Ideal for labs who want to carry out siRNA screens but lack high-throughput capabilities
The Mouse siGENOME RTF Ubiquitin Conjugation Subset 1 siRNA library serves as a preliminary evaluation of the potential role of the ubiquitin system in processes under study. Assessment of effects of Cullins, E1, and E2 enzymes will provide a reasonable idea as to whether components of the ubiquitin system are involved. Also included in this set are the HECT domain E3 ligases. Members of this relatively small family of E3s differ from the large majority of known E3s in functioning as catalytic intermediates in ubiquitin conjugation.
RTF siRNA libraries are provided as multiple single-use plate sets – just rehydrate, and add cells. This unique pre-plated format reduces hands-on time for faster screening results.
siGENOME siRNAs are designed with the proprietary SMARTselection design algorithm for high-efficiency, guaranteed silencing. They also incorporate rational strand bias with selective application of ON-TARGET modifications to optimize antisense strand loading into RISC for effective target knockdown.
Highlights
- Six ready-to-use 96-well plate sets provided for two triplicate screens
- Pre-plated, validated RNAi controls included
- No aliquoting necessary - just resuspend, and add cells
- siRNA reagents provided in clear plates at 6.25 pmol per well (50 nM final screening concentration)
- Black or white clear-bottom plates available to support assays involving fluorescent or luminescent detection
| Well | Pre-plated control | Control Catalog No. |
|---|---|---|
| A1 | siGENOME Non-targeting siRNA #2 | D-001210-02 |
| B1 | siGENOME Non-targeting siRNA #3 | D-001210-03 |
| C1 | siGENOME Non-targeting siRNA #4 | D-001210-04 |
| D1 | siGENOME Non-targeting siRNA #5 | D-001210-05 |
| E1 | siGENOME Non-targeting pool #2 | D-001206-14 |
| F1 | siGENOME Cyclophilin B control pool (H, M, R) Control siRNA | D-001136-01 |
Experimental considerations
DharmaFECT transfection reagents are highly recommended for use with RTF libraries and should be purchased separately. Refer to the DharmaFECT Cell Type Guide to find the appropriate formulation for your cell type.
Gene Targets
For a complete list of target genes in this siRNA Library, please contact Technical Support or your local Sales Representative.
For a thorough investigation of the ubiquitin pathway, you may also consider these additional libraries:
