Mouse siGENOME RTF - Ubiquitin Conjugation Subset 3
The only ready-to-use siRNA screening libraries available
The Mouse siGENOME RTF Ubiquitin Conjugation 3 siRNA library contains RING finger and RING finger-like E3 Ligases. These enzymes form a site of interaction with E2s through a platform generated as a consequence of coordination of two zinc ions. U-box E3s conform like RING finger proteins, except that they do so through salt bridges and other interactions that do not involve coordination of zinc. A20-like proteins, also included in this set, represent a class of E3 ligases, which contain a single coordination site for zinc.
RTF siRNA libraries are provided as multiple single-use plate sets – just rehydrate, and add cells. This unique pre-plated format reduces hands-on time for faster screening results.
siGENOME siRNAs are designed with the proprietary SMARTselection design algorithm for high-efficiency, guaranteed silencing. They also incorporate rational strand bias with application of ON-TARGET modifications to optimize antisense strand loading into RISC for effective target knockdown.
Highlights
- Six ready-to-use 96-well plate sets provided for two triplicate screens
- Pre-plated, validated RNAi controls included
- No aliquoting necessary, just resuspend and add cells
- siRNA reagents provided in clear plates at 6.25 pmol per well (50 nM final screening concentration)
- Black or white clear-bottom plates available to support assays involving fluorescent or luminescent detection
| Well | Pre-plated control | Control Catalog No. |
|---|---|---|
| A1 | siGENOME Non-targeting siRNA #2 | D-001210-02 |
| B1 | siGENOME Non-targeting siRNA #3 | D-001210-03 |
| C1 | siGENOME Non-targeting siRNA #4 | D-001210-04 |
| D1 | siGENOME Non-targeting siRNA #5 | D-001210-05 |
| E1 | siGENOME Non-targeting pool #2 | D-001206-14 |
| F1 | siGENOME Cyclophilin B control pool (H, M, R) Control siRNA | D-001136-01 |
Experimental considerations
DharmaFECT transfection reagents are highly recommended for use with RTF libraries and should be purchased separately. Refer to the DharmaFECT Cell Type Guide to find the appropriate formulation for your cell type.
Gene Targets
For a complete list of target genes in this siRNA Library, please contact Technical Support or your local Sales Representative.
For a thorough investigation of the ubiquitin pathway, you may also consider our three Ubiquitin Conjugation libraries:
