siGLO Green Transfection Indicator is a fluorescent oligonucleotide duplex that localizes to the nucleus, thus concentrating its signal to permit unambiguous visual assessment of uptake into mammalian cells. It is ideal for use in optimization experiments to determine optimal siRNA, crRNA:tracrRNA, or microRNA reagent transfection conditions and for monitoring relative delivery efficiency. siGLO Green (6-FAM) Transfection Indicator exhibits nearly 100% signal localization to the nucleus and represents an ideal choice for lipid-mediated transfection optimization.

Transfection Indicators are intended as a qualitative indicator of delivery and should not be used for quantitative estimation of experimental results (e.g. gene knockdown or knockout). They do not interact with RISC or Cas9 nuclease so have no gene silencing or DNA cleavage properties.

Highlights

• FAM-labeled Transfection Indicator exhibits nearly 100% signal localization to the nucleus with traditional transfection reagents
• Absorbance/Emission Max is 494/520nm, a FITC filter can be used
• Specially modified, fluorescent RNA duplex for simplified transfection assessment - without performing a functional assay
• Fluorescent signal localizes to the nucleus as an unmistakable signal of uptake
• Use as an indicator of transfection success in anyexperiment requiring synthetic RNA delivery (siRNA, crRNA:tracrRNA, sgRNA, or microRNA)