Model generation reagents

Create your own CRISPR models

Microinjection-ready reagents

Generate your own in vivo models. Access professional in vivo-grade, microinjection ready CRISPR reagents.

  • Three levels of microinjection, in vivo-grade CRISPR-Cas9 reagents
    • Basic
    • Validated
    • Validated plus
  • Prices shown are only for the sgRNA and Cas9 protein. Knock-in projects requiring a donor or additional sgRNAs will result in an increase of price.
  • Licensed by Caibou Biosciences and the Broad Institute.

Basic

Our Microinjection-ready Basic CRISPR-Cas9 reagents include an sgRNA targeting your gene (for knockout applications) and Cas9 protein, packaged separately for concentration optimization.

Included:

Price:

  • 1 aliquot sgRNA for your target gene
  • 1 aliquot Cas9, supplied as protein
  • 1 aliquot microinjection buffer
  • $990 (academic)/ $1,490 (industry)

 

Validated

All CRISPR-Cas9 reagents are validated for cutting efficiency before we inject. We send you only reagents that are proven to cut with high efficiency in an in vitro assay. We design five sgRNAs and send you the best performer.

Also, have access to our experienced design team who have designed nucleases for hundreds of mouse, rat, and rabbit projects, optimizing target sites for every application. Our team will design your sgRNAs for your specific project and application.

Included:

Price:

  • Design service-project-specific sgRNA design by our experienced scientists
  • In vitro validation of sgRNA cutting activity
  • 1 aliquot each of sgRNA for your target gene, Cas9 Protein, and Microinjection Buffer
  • Genotyping protocol and primer sequences
  • $1,990 (academic)/ $2,490 (industry)

 

Validated Plus

Our Validated+ reagents are our most comprehensive package, offering the most confidence as you generate your model. Receive our same expert design and validation services along with a detailed genotyping protocol with validated primers specifically designed for your project. Also receive validated primers for the top 10 assayable potential off-target sites for your project. These primers allow you to demonstrate the specificity of your CRISPR-Cas9 reagents and ease concerns regarding potential off-target effects.

Included:

Price:

  • Design service-project-specific sgRNA design by our experienced scientists
  • In vitro validation of sgRNA cutting activity
  • 1 aliquot each of sgRNA for your target gene, Cas9 Protein, and Microinjection Buffer
  • Validated genotyping primers and protocol
  • Validated primers for the top 10 assayable potential off-target sites
  • $3,990 (academic)/ $4,490 (industry)

 

Cas9 Accelerate Ribonucleoprotein (RNP)

Obtain animal models faster.

Bypass transcription and translation steps using Cas9 Accerlerate RNP.

Find answers to your microinjection queries

CTA Microinjection protocols

Help your microinjection success with our handy protocols