Do you recommend transfection or transduction to more precisely regulate the induceable promoter on the TRIPZ vector?
When transfecting an inducible lentiviral vector like our pTRIPZ vector, it is possible to end up with so many copies of the vector in a cell that the leakiness associated with all inducible vectors is magnified, which may mask the effect of doxycyline on the cells. It is possible to use the TRIPZ vector in a transfection based assay but you may have to optimize the amount of plasmid DNA used to minimize this effect. With transduction, you can control the number of viral particles (the MOI) going into the cells to achieve a more accurately inducible system. For the reasons mentioned above, although more precise integration can be achieved by transduction, pTRIPZ can also be transfected if preferred.