Accell Controls

Effectively optimize Accell siRNA delivery conditions and control for variables in ongoing experiments with fluorescent or unlabeled positive and negative controls. Accell Control Kits combine species-specific validated controls for assessment of Accell technology in your difficult-to-transfect cells. Kits of validated controls are available.

Experimental considerations

• Accell siRNA works at a higher concentration than conventional siRNA; recommended 1µM working concentration
• Delivery may be inhibited by the presence of BSA in serum. Optimization studies with serum-free media formulations (Accell Delivery Media) or <2.5% serum in standard media is recommended
• Full-serum media can be added back after 48 hours of incubation. Optimal mRNA silencing is typically achieved by 72 hours or up to 96 hours for protein knockdown

Choose from our selection of positive controls (targeting housekeeping genes), fluorescent or unlabeled negative controls, or an Accell Control Kit.