CRISPRmod CRISPRa synthetic crRNA non-targeting controls
Non-targeting controls to evaluate baseline cellular responses to CRISPRa components in the absence of gene target-specific crRNA
CRISPRa crRNA non-targeting controls are designed and recommended for use as negative controls for transcriptional activation experiments using CRISPRa crRNA individuals and pools. These non-targeting controls will hybridize with tracrRNA and engage the dCas9-VPR complex, but will not target any PAM-adjacent sites in the human or mouse genome. Any observed alteration in gene expression levels or viability in cells treated with these controls can be used as a baseline response of the cells to the dCas9-VPR complex for comparison to those treated with target-specific CRISPRa crRNAs and pools.
Highlights
- Proprietary alignment tools used to verify at least three mismatches or gaps to any potential target in human or mouse genomes.
- Choose an individual or pooled crRNA control to match your experimental crRNA reagent.
Please note that tracrRNA is required for use with CRISPRa synthetic crRNA reagents.
| crRNA nmol | tracrRNA nmol | 96-well plate 100 µL volume | 24-well plate 500 µL volume | 12-well plate 1000 µL volume | 6-well plate 2500 µL volume |
|---|---|---|---|---|---|
| 2 | 2 | 800 | 160 | 80 | 32 |
| 5 | 5 | 2000 | 400 | 200 | 80 |
| 10 | 10 | 4000 | 800 | 400 | 160 |
| 20 | 20 | 8000 | 1600 | 800 | 300 |
