Custom synthesis overview
Custom Synthesis Overview
|Custom Single-strand RNA|
|Single-strand RNA Synthesis||Order|
|Multiple Single-strand RNA Synthesis||Order|
|Modified siRNA Synthesis||Order|
|Multiple siRNA Syntheses||Order|
|Custom SMARTpool Design & Synthesis||Order|
|Custom microRNA||Request Quote|
|siRNA Design Tool||Design Now|
Design a single-strand RNA molecule with a wide variety of chemical modifications, learn about our capabilities for long RNA oligos, dye labeling, custom amidites and more.
Learn about customizing your siRNA with proprietary modification patterns or other chemical modifications, investigate your options for in vivo RNAi, design your own siRNA using the siDESIGN Center, learn about custom SMARTpool options and more.
Customize a miRIDIAN Mimic or Hairpin Inhibitor, or design your own microRNA reagent with specialized chemical modifications, in vivo processing, gram quantities and more.
RNA applications can be executed with greater ease and confidence by utilizing the powerful advantages of the 2'-ACE technology.
- Greater reproducibility than any other chemistry
- Crude yields of unmatched purity without additional purification time and expense
- Maximum sequence fidelity and integrity due to enhanced coupling efficiency
Download the Tech Note 2'-ACE RNA Synthesis Chemistry for more information on the details of the RNA synthesis platform.
All synthetic RNA oligos must be chemically protected at multiple sites during synthesis, regardless of the chemistry platform used. However, 2'-ACE synthesis chemistry allows for the quickest and mildest deprotection conditions compared to other chemistries like 2'-tBDMS and 2'-TOM. Short deprotection times and a mild chemical environment promote the highest level of purity for synthetic RNA in the marketplace today.
For unmodified, unpurified RNA, the following approximate percentages of full-length material can be expected:
|Product can be delivered in 2'-protected or deprotected state||Yes||No||No|
2'-ACE chemistry features superior RNA synthesis quality.
Sequence fidelity and high yields are the hallmarks of 2'-ACE chemical synthesis
Unlike other synthesis chemistries, unmodified siRNA or RNA oligos >35 nt synthesized using 2'-ACE chemistry do not require purification prior to in vitro applications.
Comparison of an unmodified, unpurified 36 nt RNA oligo synthesized using 2'-ACE, 2'-tBDMS, and 2'-TOM chemistry platforms. Anion exchange-HPLC traces for (A) 2'-ACE, (B) 2'-tBDMS, and (C) 2'-TOM illustrate a superior level of purity achieved with 2'-ACE chemistry. Smaller peaks eluting earlier than the full-length oligo (shaded area) are indicative of incomplete, non full-length, n-x products. Results are typical, but may vary slightly depending on oligo sequence.
*The average stepwise coupling efficiencies (ASCE) for each method.
**Percent full-length material listed above are approximate; exact yields will depend on sequence composition.
What is the difference between scale and yield?
- Relative amount of starting material packed into an RNA synthesis column
- Typically expressed as micromole (µmol) increments
- Final amount of material delivered
- Typically expressed as nanomole (nmol), micromole (µmol), milligram (mg), or gram (g) amounts
SCALE ≠ YIELD
Actual yields obtained for the final product may range from 10%-90% of the scale, depending on the oligo specifications (i.e. modification type, length, purification process, etc.).
If you have a minimum yield requirement for your experiments, please contact Technical Support for recommendations on which scale to select for your online order.
Available synthesis scales can accommodate microgram to multi-gram quantities
|Synthesis scales available online||Synthesis scales available by request|
|siRNA||0.025 µmol - 10 µmol||20 µmol - 2 mmol|
|Single-strand RNA||0.05 µmol - 1 µmol||2 µmol - 2 mmol|
For unmodified siRNA, the following approximate yields can be expected
|Standard (A4)**||Purified||in vivo||in vivo HPLC|
|0.025 µmol scale||20||0.25||-||-||-||-||-||-|
|0.05 µmol scale||40||0.5||20||0.25||-||-||-||-|
|0.2 µmol scale||150||2||80||1||100||1.3||50||0.65|
|0.4 µmol scale||300||4||160||2||200||2.6||100||1.3|
|1.0 µmol scale||750||10||320||4||500||6.6||200||2.6|
|2.0 µmol scale||1500||20||640||8.5||1000||13||500||6.6|
|5.0 µmol scale||3750||50||1600||21||2505||33||1000||13|
|10.0 µmol scale||7500||100||3200||42||5000||66||2500||33|
For single-strand RNA, yields may vary greatly depending on the specifications of your request (oligo length, sequence, modifications, purification, ect.). Please contact Technical Support for RNA yield estimates.
*Yields reported above should be considered estimates, and are not guaranteed amounts. Please inquire within if a minimum guaranteed amount is required.
"**Standard processing includes 2'-ACE deprotection, desalting, and annealing.
We pride ourselves on our reputation for high quality RNA synthesis.
As part of our long-standing commitment to quality, we employ the following standards for quality assurance:
- All RNA synthesis products (including siRNAs) are analyzed by mass spectrometry and quantified by UV spectroscopy
- ESI MS for all modified siRNAs and all longer or highly modified RNAs
- HPLC or PAGE for purification of RNA products
- UPLC or CGE for quality analysis when appropriate
- Custom QC methods and documentation are available upon request
See below for definitions
Quality control documentation available upon request includes:
- Mass spectrometry traces
- UPLC or CGE analysis
- Duplex or individual strand purity analysis
- Certificate of Analysis
- Endotoxin certification
- Custom QC documentation
For all mid-scale synthesis projects (200 mg - 10 g), a stringent manufacturing process is adhered to
- HPLC purification highly recommended to achieve optimal purity
- Batch records maintained on all synthesis projects (we follow GMP guidelines, but are not GMP certified)
- Endotoxin testing included on all mid-scale orders
- Data deliverables include MS, UPLC, endotoxin results, and a Certificate of Analysis
ESI MS: Electrospray ionization mass spectrometry
HPLC: High performance liquid chrmatography
PAGE: Polyacrylamide gel electrophoresis
UPLC: Ultra performance liquid chromatography
CGE: Capillary gel electrophoresis