Edit-R Synthetic sgRNA controls are recommended as positive controls for CRISPR-Cas9 experiments utilizing synthetic sgRNA to optimize transfection conditions and verify Cas9 nuclease expression.

Gene-specific positive controls and kits are designed and validated for mismatch detection assays to verify gene editing experiments. They are configured as follows:

1. Individual Edit-R synthetic sgRNA control to edit human or mouse PPIB, or DNMT3B
2. Edit-R synthetic sgRNA control kits contain the following:
• Edit-R synthetic sgRNA control (PPIB or DNMT3B)
• Mismatch detection assay forward primer (PPIB or DNMT3B) - 5 nmol
• Mismatch detection assay reverse primer (PPIB or DNMT3B) - 5 nmol

Edit-R lethal synthetic sgRNA controls are universal positive controls designed to induce cell death in a dose- and Cas9-dependent manner by targeting multiple repeat regions in the genome.

Edit-R lethal synthetic sgRNA control #1 induces very potent cell death, while lethal control #2 induces moderate cell death. These controls span a large dynamic range and allow for the optimization of CRISPR-Cas9 delivery for observation of both strong and moderate CRISPR-Cas9 phenotypes. Read the Featured Article to learn more about these controls.