CCSB Human ORFeome Gene Family Libraries

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Gene modulation
Gene overexpression
cDNA & ORFs
CCSB Human ORFeome Gene Family Libraries

CCSB Human ORFeome Gene Family Libraries

CCSB Human ORFeome Gene Family Libraries consist of Gateway-adapted Human ORFeome clones that have been arrayed into pre-defined gene family collections.

Please note that we are unable to provide additional information beyond what is listed on this page. The original source projects (e.g., MGC, ORFeome, IMAGE Consortium, DKFZ, Kazusa) no longer maintain active databases, and these legacy biological collections have limited validation. As a result, they are offered "as is" without guarantee.

To help ensure you receive the correct clone, we recommend isolating and sequencing multiple colonies (3-4) and conducting your own verification to confirm suitability for your research. Thank you for your understanding.

Derived from fully sequenced Mammalian Gene Collection (MGC) cDNAs and cloned into a Gateway entry vector at the Cancer Center for Systems Biology (CCSB) at Dana-Farber Cancer Institute, the Human ORFeome Collection is ideal for easily moving thousands of open reading frames into your choice of protein expression systems. For maximum flexibility, the native stop codon has been removed.

By organizing these clones into pre-defined gene family libraries, we provide a resource for those interested in screening or expressing large numbers of related genes.

Highlights

Popular gene family libraries enable focused screens or pathway-specific studies
Gateway entry vectors ensure easy transfer into prokaryotic, mammalian, viral, or insect expression systems
Native stop codons have been removed from the ORFs to allow for expression of fusion proteins

Gene Family	# Genes	# Clones
Cell Cycle	498	599
Cytokine	130	166
GPCR	173	198
Growth Factor	90	102
Ion Channel	133	151
Kinase	428	512
Nuclear Receptor	32	35
Oxygen Binding	26	31
Peptidase	314	369
Phosphatase	104	118
Plasma Membrane	1240	1425
Protein Kinase	271	315
Transcription Factor	415	486
Transporter	635	734
Ubiquitin	96	119

Disclaimer

Dharmacon is a distributor of multiple gene expression clone collections (cDNAs and ORFs). These clone collections were generated by groups outside of Dharmacon and thus the quality of the collections is largely dependent upon what Dharmacon received from these groups. Specific clone information and plate coordinates were provided to Dharmacon by the suppliers of these clone collections. Dharmacon has not sequence verified each individual clone from these collections. These collections and individual clones are distributed "as is" with no additional product validation or guarantees. Dharmacon has established quality procedures to ensure that individual clones are picked from the identified well in a plate, grown on the correct antibiotic, and are free of phage contamination. Due to the quality of the information provided to Dharmacon, the clone you receive might not match the expected clone. If this occurs, please contact Scientific Support Team.

Libraries are provided as glycerol stocks in 96-well microtiter plates. These ship on dry-ice via overnight delivery and should be stored at –80°C immediately upon receipt. Please contact our customer service department for a quote and estimated shipping time on library orders.