CRISPRa crRNA non-targeting controls
Non-targeting controls to evaluate baseline cellular responses to CRISPRa components in the absence of gene target-specific crRNA
CRISPRa crRNA non-targeting controls are designed and recommended for use as negative controls for transcriptional activation experiments using CRISPRa crRNA individuals and pools. These non-targeting controls will hybridize with tracrRNA and engage the dCas9-VPR complex, but will not target any PAM-adjacent sites in the human or mouse genome. Any observed alteration in gene expression levels or viability in cells treated with these controls can be used as a baseline response of the cells to the dCas9-VPR complex for comparison to those treated with target-specific CRISPRa crRNAs and pools.
Highlights
- Proprietary alignment tools used to verify at least three mismatches or gaps to any potential target in human or mouse genomes
- Choose an individual or pooled crRNA control to match your experimental crRNA reagent
Remember to order tracrRNA for use with your controls!
