Includes the E. coli Keio knockout collection, Promoter-GFP fusions for monitoring gene expression, and tagged ORFs for studying protein-protein interactions. Escherichia coli has long been a model organism for molecular genetics, with the first experiments observing bacterial conjugation occurring in the 1940's. E. coli products include collections useful for further understanding gene regulation, protein-protein interactions, and analysis of mutational effects on gene function.
E. coli Keio Knockouts
The E. coli Keio Knockout Collection was designed to provide researchers with in-frame, single-gene deletion mutants for all non-essential E. coli K-12 genes.
E. coli Promoter Collection
The E. coli Promoter Collection is a library of fluorescent reporter strains in which green fluorescent protein (GFP) is used to measure promoter activity in Escherichia coli.
E. coli Tagged ORFs
E. coli Tagged ORFs include both SPA- and TAP-tagged open reading frames intended to facilitate the mapping of protein-protein interactions in Escherichia coli.