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Species Human (hg38) Homo sapiens

Gene

Nuclease S. pyogenes nickase Cas9

PAM: NGG

Pam relative to target After

Relative to Guide 3'

Target sequence length 20

Advanced Design Options

CBE

ABE

C4-C8 for use with rAPOBEC deaminase (optimal window)

C2-C9 for use with rAPOBEC deaminase (broad window)

A5-A6 for use with ABE-exact

A4-A8 for use with ABE-flex deaminase

Splice Acceptor

Splice Donor

Premature Stop Codons

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Select Target Sequence

	Target sequence	Specificity	Genomic Location (Strand)	PAM	Targeted Region (Target base)	Type of Change	% Distance from CDS start	CDS length

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