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Species Human (hg38) Homo sapiens

Gene

Deaminase Rat Apobec

Nuclease S. pyogenes nickase Cas9

PAM: NGG

Pam relative to target After

Relative to Guide 3'

Target sequence length 20

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C2-C9

C4-C8

Splice Acceptor

Splice Donor

Premature Stop Codons

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	Target sequence	Specificity	Genomic Location (Strand)	Targeted Region (Target base)	Type of Change	% Distance from CDS start	CDS length		PAM