Multiplex I cfDNA Reference Standard Set
The Multiplex I cfDNA Reference Standards are highly-characterized, biologically-relevant reference materials used to assess the performance of cfDNA assays that detect somatic mutations, including quantitative PCR, next-generation sequencing, and other novel assays
High throughput platforms such as Next generation sequencing (NGS) and quantitative PCR (qPCR) platforms give researchers much greater power when profiling cfDNA samples. However, the use of these presents challenges surrounding sample variability, platform bias, and potential failure to detect biomarkers. Horizon’s cfDNA products are all derived from human cell lines, and are fragmented to an average size of 160 bp to closely resemble cfDNA extracted from human plasma.
This format ensures that they may applied to a wide-range of cfDNA NGS and qPCR assays including high-throughput platforms such as NGS and qPCR give researchers greater power when profiling cfDNA samples. Horizon has developed the cfDNA Reference Standard range to support the development and continued validation of cfDNA assays.
The Multiplex I cfDNA Reference Standard Set covers multiple engineered single nucleotide variants (SNVs/SNPs) with 8 mutations at 5%, 1%, and 0.1% allelic frequencies. Batch-specific copy number values measured via ddPCR are provided with each Set.
With this product you are able to:
- Analyze the sensitivity and specificity of your assay
- Gain certainty of the limit of detection and limit of quantification
- Optimize and validate new cancer panels and routinely monitor the performance of your assay
Technical Data
Format
cfDNA
Genes Covered
EGFR, KRAS, NRAS, PI3KCA
Cosmics
1,500+
Allelic Frequencies
5%, 1%, 0.1% and 0% (100% wild type)
Buffer
Tris-EDTA (10mM Tris-HCl, 1mM EDTA), pH 8.0
Product Information
Fragment Size
160 bp
Unit Size
350 ng per vial
Concentration
20 ng/µl
Verified Mutations
100% Multiplex I Wild Type cfDNA Reference Standard (Part No.: HD776) |
|||
Chromosome |
Gene |
Variant |
Expected Allelic Frequency |
7p12 |
EGFR |
L858R |
0.00% |
7p12 |
EGFR |
ΔE746 - A750 |
0.00% |
7p12 |
EGFR |
T790M |
0.00% |
7p12 |
EGFR |
V769 - D770insASV |
0.00% |
12p12.1 |
KRAS |
G12D |
0.00% |
1p13.2 |
NRAS |
Q61K |
0.00% |
1p13.2 |
NRAS |
A59T |
0.00% |
3q26.3 |
PIK3CA |
E545K |
0.00% |
5% Multiplex I cfDNA Reference Standard (Part No.: HD777) |
|||
Chromosome |
Gene |
Variant |
Expected Allelic Frequency |
7p12 |
EGFR |
L858R |
5.0% |
7p12 |
EGFR |
ΔE746 - A750 |
5.0% |
7p12 |
EGFR |
T790M |
5.0% |
7p12 |
EGFR |
V769 - D770insASV |
5.0% |
12p12.1 |
KRAS |
G12D |
6.3% |
1p13.2 |
NRAS |
Q61K |
6.3% |
1p13.2 |
NRAS |
A59T |
6.3% |
3q26.3 |
PIK3CA |
E545K |
6.3% |
1% Multiplex I cfDNA Reference Standard (Part No.: HD778) |
|||
Chromosome |
Gene |
Variant |
Expected Allelic Frequency |
7p12 |
EGFR |
L858R |
1.0% |
7p12 |
EGFR |
ΔE746 - A750 |
1.0% |
7p12 |
EGFR |
T790M |
1.0% |
7p12 |
EGFR |
V769 - D770insASV |
1.0% |
12p12.1 |
KRAS |
G12D |
1.3% |
1p13.2 |
NRAS |
Q61K |
1.3% |
1p13.2 |
NRAS |
A59T |
1.3% |
3q26.3 |
PIK3CA |
E545K |
1.3% |
0.1% Multiplex I cfDNA Reference Standard (Part No.: HD779) |
|||
Chromosome |
Gene |
Variant |
Expected Allelic Frequency |
7p12 |
EGFR |
L858R |
0.10% |
7p12 |
EGFR |
ΔE746 - A750 |
0.10% |
7p12 |
EGFR |
T790M |
0.10% |
7p12 |
EGFR |
V769 - D770insASV |
0.10% |
12p12.1 |
KRAS |
G12D |
0.13% |
1p13.2 |
NRAS |
Q61K |
0.13% |
1p13.2 |
NRAS |
A59T |
0.13% |
3q26.3 |
PIK3CA |
E545K |
0.13% |
Presence confirmed in parental cell line
EGFR G719S (20% allelic frequency); download additional information on variants present in the parental cell lines:
General Information
Storage
4°C
Expiry
See all product shelf life information
Cell Line Background
RKO/SW48
Quality Control
Fragmentation Size
D1000 DNA ScreenTape assay
Allelic Frequency
Droplet Digital™ PCR
Quantification
Qubit dsDNA BR Assay (post-fragmentation)
Please see our terms and conditions of ordering.
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- Product code(s) of the item(s) you wish to order (e.g. "HD123")
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Shipping Information
Shipping information for all territories can be found on our Shipping Charges page. This includes Shipping and Handling fees, as well as shipping conditions for each product range.
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