Tru-Q 2 (5% Tier) Reference Standard

The Tru-Q DNA Reference Standards are a highly-characterized, biologically-relevant quality control material used to assess the performance of NGS assays that detect somatic mutations. The current Batch of HD729 SNB 40755 has been released on concession and is the only batch available for purchase. To view the details of the concession before you purchase, please click here. The specifications of some variants within the Tru-Q product line have been changed for batches manufactured after the 31st March 2021. For more information, please click here.

Next generation sequencing (NGS) platforms give researchers much greater power when profiling tumor samples. However, the use of NGS presents challenges surrounding sample variability, platform bias, and potential failure to detect biomarkers. Horizon’s standards are appropriate for any NGS library preparation including whole-genome, whole-exome, custom capture and targeted amplicon panels. Horizon has developed this Quality-Seq (Q-Seq) NGS Reference Standard range to support the development and continued validation of Next Generation Sequencing platforms. The Tru-Q DNA Reference Standard portfolio covers multiple endogenous SNPs, insertions and deletions. Tru-Q NGS DNA Reference Standard 1 (5% Tier) covers 10 mutations at 5% allelic frequency in genomic DNA format. These may be diluted to even lower allelic frequencies using our Tru-Q 0 Wild Type standard. Furthermore, because the Tru-Q series has 4 different standards at the 5% alleleic frequency range, you may rotate these as “blinded” samples for proficiency testing in your laboratory. These standards are available only in gDNA format.

With this product you are able to:

  • Analyze the sensitivity and specificity of your assay
  • Gain certainty of the limit of detection and limit of quantification
  • Optimize and validate new cancer panels and routinely monitor the performance of your assay

 

Technical Data

Format: Genomic DNA

Genes Covered: ALK, BRAF, EGFR, FGFR2, GNAQ, IDH2, KRAS, NRAS, PIK3CA

Allelic Frequencies: 5% for verified variants

Buffer: Tris-EDTA (10mM Tris-HCl, 1mM EDTA), pH 8.1

 

Product Information

Verified Mutations: The Tru-Q Reference Standard has been manufactured using multiple engineered cell lines and mixed to generate multiple Allelic Frequencies in multiplex. An example of the allelic frequency by whole-exome sequencing for one batch of this product is reported in the table below.

Gene Variant (AA) CDS mutation GRCh38 co-ordinates Expected allelic frequency (%) NGS allelic frequency (%) NGS read depth
ALK F1174L c.3522C>A 2:29220829 5.00% 3.43% 496
BRAF V600E c.1799T>A 7:140753336 7.70% 6.70% 702
BRAF V600G c.1799T>G 7:140753336 3.80% a a
EGFR G719S c.2155G>A 7:55174014 16.70% 15.75% 616
EGFR L858R c.2573T>G 7:55191822 4.20% 3.82% 314
FGFR2 S252W c.755C>G 10:121520163 4.00% 3.35% 716
GNAQ Q209L c.626A>T 9:77794572 5.00% 4.59% 610
IDH2 R140Q c.419G>A 15:90088702 5.00% 4.92% 590
KRAS G12V c.35G>T 12:25245350 5.00% 5.16% 465
KRAS G13D c.38G>A 12:25245347 25.00% 23.77% 467
KRAS Q61L c.182A>T 12:25227342 5.00% 5.74% 749
NRAS Q61L c.182A>T 1:114713908 5.00% 6.02% 664
PIK3CA E545K c.1633G>A 3:179218303 5.00% 5.06% 1127
PIK3CA H1047R c.3140A>G 3:179234297 30.00% 27.39% 825

a – Not detected - did not meet detection threshold, below LOD

Unit Size: 1 µg

Concentration: 50 ng/µl

 

General Information

Storage: 4˚C

Expiration: See all product shelf life information

 

Quality Control

Allelic Frequency: Droplet Digital PCR™

DNA Integrity: Agarose gel electrophoresis

DNA Quantification: Spectrophotometry (A260)